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Type: Journal article
Title: Activation of the gut-associated lymphoid tissue with expression of interleukin-2 receptors that peaks during weaning in the rat
Author: Masjedi, M.
Tivey, D.
Thompson, F.
Cummins, A.
Citation: Journal of Pediatric Gastroenterology and Nutrition, 1999; 29(5):556-562
Issue Date: 1999
ISSN: 0277-2116
Statement of
Masjedi, Mohsen; Tivey, David R.; Thompson, Fiona M.; Cummins, Adrian G.
Abstract: BACKGROUND:Weaning exposes the intestinal mucosa to food and bacterial antigens at an age when the immune system is believed to be immature and functionally defective. The purpose of this study was to investigate changes in activation and phenotype of immune cells of the gut-associated lymphoid tissue during weaning. METHODS:Litters of infant rats were studied from pre- to postweaned life. The activation status, assessed by interleukin-2 receptor (IL-2R) expression, and phenotype of cells in the gut-associated lymphoid tissue were examined by immunostaining. RESULTS:Interleukin-2 receptor expression peaked two to four-fold at midweaning (day 21) in mesenteric lymph nodes, jejunal lamina propria, Peyer's patches, and intraepithelial lymphocytes, compared with adult animals (day 70). CD45+ cells expanded in the lamina propria, epithelium, and lymphocyte-filled villi. With CD45 as the denominator, 10% to 50% of lymphocytes in the lamina propria and epithelium were alphabetaT-cell receptor (TCR)+, but the remaining cells had a null phenotype, because there were low numbers of gammadeltaTCR+ T cells, B cells, and macrophages. Natural killer cells peaked at midweaning in the lamina propria (9%) and epithelium (20%) but were less than 5% of CD45+ cells after weaning. CONCLUSIONS:Rather than being immature or functionally inactive, the gut-associated lymphoid tissue reacts appropriately during weaning with expression of IL-2R and expansion of alphabetaTCR+ T-cells.
Keywords: Gut-associated lymphoid tissue, Interleukin-2 receptor, Intraepithelial lymphocytes, Lamina propria cells, Lymphocyte-filled villi, T cell, Weaning
Rights: © 1999 Lippincott Williams & Wilkins, Inc.
DOI: 10.1097/00005176-199911000-00016
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