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Type: Journal article
Title: A gene targeting suicide vector for Streptococcus bovis
Author: Brooker, J.
Lum, D.
Thomson, A.
Ward, H.
Citation: Letters in Applied Microbiology, 1995; 21(5):292-297
Publisher: Society for Applied Bacteriology
Issue Date: 1995
ISSN: 0266-8254
Abstract: A gene-targeting suicide vector for Streptococcus bovis has been constructed using the Escherichia coli/Streptococcus shuttle plasmid, pMU1328, and a region derived from the broad host-range, Gram-positive transposon, Tn916. This suicide plasmid replicates autonomously in E. coli, but not in Strep. bovis or Strep. bovis Tn916. Under positive selection, the plasmid was shown to integrate into Strep. bovis Tn916 chromosomal DNA at a frequency of 3 x 10(-8) cell-1 and was stably maintained for at least 100 generations in the absence of selection. This is the first report of a recombination system in ruminal bacteria. The ability to target genes, knock out specific functions or introduce novel genes into these micro-organisms will allow ruminal species to be manipulated and may eventually lead to improved animal production.
Keywords: Escherichia coli; Streptococcus bovis; Bacterial Proteins; DNA, Bacterial; DNA Transposable Elements; Gene Targeting; Transformation, Bacterial; Tetracycline Resistance; DNA Replication; Genetic Vectors
RMID: 0030003759
DOI: 10.1111/j.1472-765X.1995.tb01063.x
Appears in Collections:Agriculture, Food and Wine publications

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