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Type: Journal article
Title: Development and evaluation of a custom bait design based on 469 single-copy protein-coding genes for exon capture of isopods (Philosciidae: Haloniscus)
Author: Stringer, D.
Bertozzi, T.
Meusemann, K.
Delean, J.
Guzik, M.
Tierney, S.
Mayer, C.
Cooper, S.
Zwick, A.
Austin, A.
Citation: PLoS One, 2021; 16(9):1-24
Publisher: Public Library of Science
Issue Date: 2021
ISSN: 1932-6203
Editor: ZHANG, F.
Statement of
Danielle N. Stringer, Terry Bertozzi, Karen Meusemann, Steven Delean, Michelle T. Guzik, Simon M. Tierney ... et al.
Abstract: Transcriptome-based exon capture approaches, along with next-generation sequencing, are allowing for the rapid and cost-effective production of extensive and informative phylogenomic datasets from non-model organisms for phylogenetics and population genetics research. These approaches generally employ a reference genome to infer the intron-exon structure of targeted loci and preferentially select longer exons. However, in the absence of an existing and well-annotated genome, we applied this exon capture method directly, without initially identifying intron-exon boundaries for bait design, to a group of highly diverse Haloniscus (Philosciidae), paraplatyarthrid and armadillid isopods, and examined the performance of our methods and bait design for phylogenetic inference. Here, we identified an isopod-specific set of single-copy protein-coding loci, and a custom bait design to capture targeted regions from 469 genes, and analysed the resulting sequence data with a mapping approach and newly-created post-processing scripts. We effectively recovered a large and informative dataset comprising both short (<100 bp) and longer (>300 bp) exons, with high uniformity in sequencing depth. We were also able to successfully capture exon data from up to 16-year-old museum specimens along with more distantly related outgroup taxa, and efficiently pool multiple samples prior to capture. Our well-resolved phylogenies highlight the overall utility of this methodological approach and custom bait design, which offer enormous potential for application to future isopod, as well as broader crustacean, molecular studies.
Keywords: Animals
Genetics, Population
Gene Expression
Open Reading Frames
Genetic Loci
High-Throughput Nucleotide Sequencing
Arthropod Proteins
Datasets as Topic
Rights: © 2021 Stringer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0256861
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Appears in Collections:Environment Institute publications

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