Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/13409
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Type: Journal article
Title: Development of a PCR-based allele-specific assay from an RFLP probe linked to resistance to cereal cyst nematode in wheat
Author: Williams, Kevin J.
Fisher, John M.
Langridge, Peter
Citation: Genome, 1996; 39(4):798-801
Publisher: National Research Council of Canada
Issue Date: 1996
ISSN: 0831-2796
Statement of
Responsibility: 
Kevin J. Williams, John M. Fisher and Peter Langridge
Abstract: The RFLP locus Xglk605 identified by the probe Tag605 maps to a proximal position on the long arm of wheat chromosome 2B about 7 cM away from a gene conditioning resistance to cereal cyst nematode in the wheat line AUS10894. The clone Tag605 was partially sequenced and the PCR primer set AWP1 was designed. The 292-bp product, which showed no polymorphism between varieties, was cloned and sequenced. A single base difference was found in the sequence of the AWP1 products amplified and cloned from the wheat lines AUS10894 and 'Spear'. PCR primers were designed with 3′ termini that corresponded to the two alleles. A dual-PCR system was developed in which the primer sets AWP2 and AWP3 produced allele-specific amplification. The concentration of the oligonucleotide primers and the sequence of the primer–template mismatches were critical to the success of discriminatory allele amplification. Key words : Triticum aestivum, STS, cereal cyst nematode, RFLP.
Keywords: Triticum aestivum; STS; cereal cyst nematode; RFLP
Rights: Copyright status unknown
DOI: 10.1139/g96-100
Appears in Collections:Agriculture, Food and Wine publications

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