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https://hdl.handle.net/2440/13557
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Type: | Journal article |
Title: | Molecular cloning of a cDNA encoding a (1→4)-β-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum) |
Other Titles: | Molecular cloning of a cDNA encoding a (1->4)-beta-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum) |
Author: | Bewley, J. Burton, R. Morohashi, Y. Fincher, G. |
Citation: | Planta: an international journal of plant biology, 1997; 203(4):454-459 |
Publisher: | SPRINGER |
Issue Date: | 1997 |
ISSN: | 0032-0935 1432-2048 |
Statement of Responsibility: | J. Derek Bewley, Rachel A. Burton, Yukio Morohashi, Geoffrey B. Fincher |
Abstract: | Mannose-containing polysaccharides are widely distributed in cell walls of higher plants. During endosperm mobilization in germinated tomato seeds (1-->4)-beta-mannan endohydrolases (EC 3.2.1.78) participate in the enzymic depolymerization of these cell wall polysaccharides. A cDNA encoding a (1-->4)-beta-mannanase from the endosperm of germinated tomato (Lycopersicon esculentum Mill.) seeds has been isolated and characterized. The amino acid sequence deduced from the 5'-region of the cDNA exactly matches the sequence of the 65 NH2-terminal amino acids determined directly from the purified enzyme. The mature enzyme consists of 346 amino acid residues, it has a calculated M(r) of 38,950 and an isoelectric point of 5.3. Overall, the enzyme exhibits only 28-30% sequence identity with fungal (1-->4)-beta-mannanases, but more highly conserved regions, which may represent catalytic and substrate-binding domains, can be identified. Based on classification of the tomato (1-->4)-beta-mannanase as a member of the family 5 group of glycosyl hydrolases, Glu-148 and Glu-265 would be expected to be the catalytic acid and the catalytic nucleophile, respectively. Southern hybridization analyses indicate that the enzyme is derived from a family of about four genes. Expression of the genes, as determined by the presence of mRNA transcripts in Northern hybridization analyses, occurs in the endosperm of germinated seeds; no transcripts are detected in hypocotyls, cotyledons, roots or leaves. |
Keywords: | Cell wall endosperm mobilization gene expression patterns lycopersicon (1→4)-β-Mannan endohydrolase (primary structure) polysaccharide |
Rights: | © Springer-Verlag 1997 |
DOI: | 10.1007/s004250050214 |
Published version: | http://dx.doi.org/10.1007/s004250050214 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 2 |
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