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|Title:||Development of YLM, a codominant PCR marker closely linked to the Yd2 gene for resistance to barley yellow dwarf disease|
|Citation:||Theoretical and Applied Genetics: international journal of plant breeding research, 1998; 96(8):1170-1177|
|Paltridge, N. G. ; Collins, N. C. ; Bendahmane, A. ; Symons, R. H.|
|Abstract:||The Yd2 gene in barley provides protection against barley yellow dwarf luteovirus (BYDV), the most economically devastating virus of cereals worldwide. Because resistance assays to identify Yd2-containing individuals from breeding populations are often difficult, we have developed a closely linked, codominant PCR-based marker for Yd2 using AFLP marker technology. The marker, designated YLM, can be amplified from barley genomic DNA prepared using a rapid and simple extraction procedure and, in a survey of more than 100 barley genotypes, was found to be polymorphic between most Yd2 and non-Yd2 lines. The YLM therefore shows excellent potential as a tool for selecting Yd2-carrying segregants in barley breeding programmes.|
|Appears in Collections:||Agriculture, Food and Wine publications|
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