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dc.contributor.authorTan, J.P.-
dc.contributor.authorLiu, X.-
dc.contributor.authorPolo, J.M.-
dc.identifier.citationNature Protocols, 2022; 17(12):2739-2759-
dc.descriptionPublished online: 14 October 2022.-
dc.description.abstractDuring early mammalian embryonic development, trophoblast cells play an essential role in establishing cell-cell interactions at the maternal-fetal interface to ensure a successful pregnancy. In a recent study, we showed that human fibroblasts can be reprogrammed into induced trophoblast stem (iTS) cells by transcription factor-mediated nuclear reprogramming using the Yamanaka factors OCT4, KLF4, SOX2 and c-MYC (OKSM) and a selection of TS cell culture conditions. The derivation of TS cells from human blastocysts or first-trimester placenta can be limited by difficulties in obtaining adequate material as well as ethical implications. By contrast, the described approach allows the generation of iTS cells from the adult cells of individuals with diverse genetic backgrounds, which are readily accessible to many laboratories around the world. Here we describe a step-by-step protocol for the generation and establishment of human iTS cells directly from dermal fibroblasts using a non-integrative reprogramming method. The protocol consists of four main sections: (1) recovery of cryopreserved human dermal fibroblasts, (2) somatic cell reprogramming, (3) passaging of reprogramming intermediates and (4) derivation of iTS cell cultures followed by routine maintenance of iTS cells. These iTS cell lines can be established in 2-3 weeks and cultured long term over 50 passages. We also discuss several characterization methods that can be performed to validate the iTS cells derived using this approach. Our protocol allows researchers to generate patient-specific iTS cells to interrogate the trophoblast and placenta biology as well as their interactions with embryonic cells in health and diseases.-
dc.description.statementofresponsibilityJia Ping Tan, Xiaodong Liu and Jose M. Polo-
dc.publisherSpringer Nature-
dc.rightsCopyright © 2022, Springer Nature Limited. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.-
dc.subjectCells, Cultured-
dc.subjectCell Culture Techniques-
dc.subjectCell Differentiation-
dc.subjectOctamer Transcription Factor-3-
dc.subjectKruppel-Like Transcription Factors-
dc.subjectSOXB1 Transcription Factors-
dc.subjectInduced Pluripotent Stem Cells-
dc.subjectCellular Reprogramming-
dc.titleEstablishment of human induced trophoblast stem cells via reprogramming of fibroblasts-
dc.typeJournal article-
dc.identifier.orcidPolo, J.M. [0000-0002-2531-778X]-
Appears in Collections:Medicine publications
South Australian Immunogenomics Cancer Institute (SAIGENCI) publications

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