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Type: Journal article
Title: The function of the Medicago truncatula ZIP transporter MtZIP14 is linked to arbuscular mycorrhizal fungal colonisation
Author: Watts-Williams, S.J.
Wege, S.
Ramesh, S.A.
Berkowitz, O.
Xu, B.
Gilliham, M.
Whelan, J.
Tyerman, S.D.
Citation: Plant, Cell and Environment, 2023; 46(5):1691-1704
Publisher: Wiley
Issue Date: 2023
ISSN: 0140-7791
Statement of
Stephanie J. Watts, Williams, Stefanie Wege, Sunita A. Ramesh, Oliver Berkowitz, Bo Xu, Matthew Gilliham, James Whelan, Stephen D. Tyerman
Abstract: Soil micronutrient availability, including zinc (Zn), is a limiting factor for crop yield. Arbuscular mycorrhizal (AM) fungi can improve host plant growth and nutrition through the mycorrhizal pathway of nutrient uptake. Although the physiology of Zn uptake through the mycorrhizal pathway is well established, the identity of the related molecular components are unknown. Here, RNA-seq analysis was used to identify genes differentially-regulated by AM colonisation and soil Zn concentration in roots of Medicago truncatula. The putative Zn transporter gene MtZIP14 was markedly up-regulated in Medicago truncatula roots when colonised by Rhizophagus irregularis. MtZIP14 restored yeast growth under low Zn availability. Loss-of-function mutant plants (mtzip14) had reduced shoot biomass compared to the wild-type when colonised by AM fungi and grown under low and sufficient soil Zn concentration; at high soil Zn concentration, there were no genotypic differences in shoot biomass. Vesicular and arbuscular colonisation of roots was lower in the mtzip14 plants regardless of soil Zn concentration. We propose that MtZIP14 is linked to AM colonisation in M. truncatula plants, with the possibility that MtZIP14 function with AM colonisation is linked to soil or plant Zn nutrition. This article is protected by copyright. All rights reserved.
Keywords: Arbuscular mycorrhizal fungi
nutrient uptake
Description: First published: 18 January 2023
Rights: © 2023 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
DOI: 10.1111/pce.14545
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Appears in Collections:Agriculture, Food and Wine publications

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