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Type: Journal article
Title: Determination of perhexiline and hydroxyperhexiline in plasma by liquid chromatography-mass spectrometry
Author: Beck, O.
Stephanson, N.
Morris, R.
Sallustio, B.
Hjemdahl, P.
Citation: Journal of Chromatography. B, Analytical Techniques in the Biomedical and Life Sciences, 2004; 805(1):87-91
Publisher: Elsevier Science BV
Issue Date: 2004
ISSN: 1570-0232
Abstract: A method for the quantitative determination of perhexiline and its main hydroxylated metabolites in human plasma, based on liquid chromatography-mass spectrometry (LC-MS), was developed. The method used protein precipitation with acetonitrile followed by dilution with water and subsequent direct injection of the extract into the LC-MS system. Hexadiline was used as internal standard and the intra-assay coefficients of variation were <or=5% for perhexiline and cis-hydroxyperhexiline over the target concentration range in patients. The lower limits of quantification were 0.005mg/l for perhexiline and 0.015mg/l for cis-hydroxyperhexiline, and the measuring ranges were from 0.05 to 3.0 and from 0.2 to 6.0mg/l, respectively. The method was compared with an established HPLC method with fluorescence detection and the correlation between the methods was close to 1 for both compounds. The predominant form of hydroxyperhexiline in 87% of the patient samples was found to be one of the diastereomeric pairs of cis-hydroxyperhexiline. In patients not forming this metabolite, trans-hydroxyperhexiline could be detected. We conclude that the present LC-MS method is suitable for use in a clinical routine laboratory.
Keywords: Humans; Perhexiline; Cardiovascular Agents; Chromatography, High Pressure Liquid; Isomerism; Reference Standards; Mass Spectrometry
RMID: 0020040415
DOI: 10.1016/j.jchromb.2004.02.019
Appears in Collections:Pharmacology publications

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