Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/14554
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Type: Journal article
Title: Dietary polyunsaturated fatty acid and antioxidant modulation of vascular dysfunction in the spontaneously hypertensive rat
Author: Abeywardena, M.
Head, R.
Citation: Prostaglandins, Leukotrienes and Essential Fatty Acids, 2001; 65(2):91-97
Publisher: Churchill Livingstone
Issue Date: 2001
ISSN: 0952-3278
1532-2823
Statement of
Responsibility: 
M.Y. Abeywardena and R.J. Head
Abstract: Two currently available edible oils–olive and canola–and two oil blends of plant origin having different n-3/n-6 polyunsaturated fatty acid (PUFA) ratios were evaluated for their ability to modify vascular dysfunction in the spontaneously hypertensive rat (SHR). Synthetic diets supplemented with test oils (5% w/w) were fed for 12 weeks, and segments of thoracic aorta used to assess vascular function. Vessels from the SHR displayed a spontaneous constrictor response after the inhibition of endothelial cell nitric oxide (NO) with Nω-nitro-L-arginine (NOLA). Dietary α -linoleate enrichment led to a reduction (P<0.05) in this abnormality with a dietary n-3/n-6 PUFA ratio of 1.0 (blend-1) yielding the best outcome. Relaxation to acetylcholine (ACh) was unaffected by dietary lipid supplementation. NOLA treated rings also displayed contractions to ACh that were abolished by indomethacin, thromboxane antagonists SQ29548, picotamide and flavonoids kaempferol and quercetin. In contrast, α -tocopherol, rutin and the lipoxygenase inhibitor esculetin resulted in only partial (30–55%) inhibition, and were ineffective against the NOLA-induced contraction suggesting the operation of different biochemical mechanisms in mediating the spontaneous and Ach-induced contractions. Results implicate plant-based oils and antioxidants as potential modulators of vascular function.
Keywords: Aorta
Endothelium, Vascular
Animals
Rats, Inbred SHR
Rats
Hypertension
Nitric Oxide
Acetylcholine
Phthalic Acids
Hydrazines
Flavonoids
Kaempferols
Quercetin
Rutin
Umbelliferones
Indomethacin
Fatty Acids, Unsaturated
Plant Oils
alpha-Tocopherol
Cardiovascular Agents
Platelet Aggregation Inhibitors
Antioxidants
Diet
Dose-Response Relationship, Drug
Lipid Metabolism
Bridged Bicyclo Compounds, Heterocyclic
Rights: © 2001 Harcourt Publishers Ltd
DOI: 10.1054/plef.2001.0294
Published version: http://dx.doi.org/10.1054/plef.2001.0294
Appears in Collections:Aurora harvest 2
Pharmacology publications

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