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Type: Journal article
Title: The influence of intracellular polyglucose and prior growth rate on the survival of Fusobacterium nucleatum under starvation conditions
Author: Rogers, A.
Zilm, P.
Citation: Molecular Oral Microbiology, 1995; 10(2):119-121
Publisher: Munksgaard
Issue Date: 1995
ISSN: 0902-0055
Abstract: Grown in a chemically defined medium containing glucose at a dilution rate of D = 0.065 h-1, Fusobacterium nucleatum D212B-2 produced large amounts of intracellular polyglucose. Aliquots of this culture were starved by anaerobic incubation at 37 degrees C and at various times, assayed for intracellular polyglucose content and viability. This protocol was repeated using cells grown under the same conditions in a chemically defined medium, a medium lacking carbohydrate and in which the organism produced no intracellular polyglucose. Both cultures had 50% survival time values of about 1.5 h and were not eliminated even after 32 h of starvation. It was, therefore concluded that starvation-survival is not influenced by intracellular polyglucose. Starvation-survival was also determined for cells grown in a chemically defined medium at D = 0.048 h-1 and D = 0.12 h-1. The faster-grown cells had a 50% survival time of 3.8 h but were completely eliminated after 8-16 h of starvation. In contrast, slower-grown cells had a 50% survival time of 1.5 h but were not completely eliminated after 32 h of starvation. This illustrates the importance of cell history and technique standardization in comparing the starvation-survival of different organisms.
Keywords: Fusobacterium nucleatum
Culture Media
Bacteriological Techniques
Adaptation, Physiological
Cell Division
DOI: 10.1111/j.1399-302X.1995.tb00130.x
Appears in Collections:Aurora harvest 2
Dentistry publications

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