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Type: Journal article
Title: Birth of pouch young after artificial insemination in the tammar wallaby (Macropus eugenii)
Author: Paris, D.
Taggart, D.
Shaw, G.
Temple-Smith, P.
Renfree, M.
Citation: Biology of Reproduction, 2005; 72(2):451-459
Publisher: Soc Study Reproduction
Issue Date: 2005
ISSN: 0006-3363
Statement of
Damien B.B.P. Paris; David A. Taggart; Geoff Shaw; Peter D. Temple-Smith and Marilyn B. Renfree
Abstract: Timing of artificial insemination (AI) in marsupials is critical because fertilization must occur before mucin coats the oocyte during passage through the oviduct. In this study, timing and the site of insemination were examined to develop AI in the tammar wallaby (Macropus eugenii). Birth and postpartum (p.p.) estrus was synchronized in 46 females. Epididymal spermatozoa (n=4) or semen collected by electroejaculation (n=42) were inseminated early (4-21 h p.p.) into the urogenital sinus (n=7), the anterior vaginal culs de sac (n=7), the uterus by transcervical catheter (n=5), or the uterus by injection (intrauterine artificial insemination, IUAI) (n=5). A further 16 females were inseminated late (19-48 h p.p.) by IUAI. All females were monitored for birth. A third group of six females was inseminated late (21-54 h p.p.) by IUAI and 0.4-6.6 h later, sperm had reached the oviduct in all animals. In total, an oocyte to which spermatozoa were attached was recovered and two young were born after IUAI using epididymal (n=1) or electroejaculated (n=2) spermatozoa, but no young resulted from insemination at other sites. Two females were successfully inseminated at 43 and 47 h p.p., later than most other animals, and the third was inseminated much earlier (18 h p.p.) but with highly motile spermatozoa. These young represent the first macropodids born by AI and the first marsupials conceived using epididymal spermatozoa.
Keywords: Vagina
Animals, Newborn
Culture Media
Semen Preservation
Insemination, Artificial
Electric Stimulation
Estrus Synchronization
Ovum Transport
Microsatellite Repeats
DOI: 10.1095/biolreprod.104.033282
Appears in Collections:Aurora harvest 2
Earth and Environmental Sciences publications

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