Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Production of osteopontin by cultured porcine epithelial cell rests of Malassez
Author: Rincon, J.
Xiao, Y.
Young, W.
Bartold, P.
Citation: Journal of Periodontal Research, 2005; 40(5):417-426
Publisher: Blackwell Munksgaard
Issue Date: 2005
ISSN: 0022-3484
Statement of
Julio C. Rincon, Yin Xiao, William G. Young and P. Mark Bartold
Abstract: Background: The epithelial cell rests of Malassez (ERM) are derived from Hertwig’s epithelial root sheath (HERS). During development the cells of HERS deposit a variety of molecules on the newly forming root surface. The possibility that ERM retain this potential after root development is completed and secrete bone or cementum-related proteins needs to be investigated. The purpose of this study was to determine the expression of the non-collagenous proteins osteopontin (OPN) and bone sialoprotein (BSP) by cells derived from the epithelial cell rests of porcine periodontium. Methods: ERM and fibroblasts were cultured from porcine periodontal ligament. The cells were identified and characterized using transmission electron microscopy, immunohistochemistry, western blot analysis of proteins, reverse transcription– polymerase chain reaction and ability to form mineralized nodules in culture. In particular the expression of the mineralized tissue-related proteins, BSP and OPN, was studied. Results: Cells from porcine periodontal ligaments were successfully cultured; separated and characterized as being of either an epithelial or fibroblastic phenotype. Although the ERM did not form mineralized nodules in culture, they did express a significant amount of mRNA for OPN. Conclusion: The results from this study provide evidence that ERM express mRNA for at least one bone/cementum-related protein. Whether this function would be consistent with a role for ERM in tissue formation, inflammation and regeneration remains to be established.
Keywords: bone
epithelial cell rests of Malassez
Description: The definitive version is available at
DOI: 10.1111/j.1600-0765.2005.00823.x
Appears in Collections:Aurora harvest 2
Dentistry publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.