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Type: Journal article
Title: Identification of the causal agent of pistachio dieback in Australia
Author: Facelli, E.
Taylor, C.
Scott, E.
Fegan, M.
Huys, G.
Noble, R.
Swings, J.
Sedgley, M.
Citation: European Journal of Plant Pathology, 2005; 112(2):155-165
Publisher: Kluwer Academic Publ
Issue Date: 2005
ISSN: 0929-1873
Statement of
E. Facelli, C. Taylor, E. Scott, M. Fegan, G. Huys, R. D. Noble, J. Swings and M. Sedgley
Abstract: Symptoms associated with pistachio dieback in Australia include decline (little or no current season growth), xylem staining in shoots two or more years old, trunk μ and limb lesions (often covered by black, superficial fungal growth), excessive exudation of resin, dieback and death of the tree. Bacteria belonging to the genus Xanthomonas have been suggested as the causal agent. To confirm the constant association between these bacteria and the disease syndrome, the absence of other pathogens and the identity of the pathogen, we performed a series of isolations and pathogenicity tests. The only microorganism consistently isolated from diseased tissue was a bacterium that produced yellow, mucoid colonies and displayed morphological and cultural characteristics typical of the genus Xanthomonas. Database comparisons of the fatty acid and whole-cell protein profiles of five representative pistachio isolates indicated that they all belonged to X. translucens, but it was not possible to allocate the isolates to pathovar. Pathogenicity tests on cereals and grasses supported this identification. However, Koch’s postulates have been only partially fulfilled because not all symptoms associated with pistachio dieback were reproduced on inoculated twoyear- old pistachio trees. While discolouration was observed, dieback, excessive resinous exudate and trunk and limb lesions were not produced; expression of these symptoms may be delayed, and long-term monitoring of a small number of inoculated trees is in progress.
Keywords: Fatty acid methyl esters (FAME) analysis; rep-PCR; whole-cell protein profiling; Xanthomonas translucens
Description: The original publication can be found at
RMID: 0020050473
DOI: 10.1007/s10658-005-3120-9
Appears in Collections:Agriculture, Food and Wine publications

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