Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||A survey of glycosidase activities of commercial wine strains of Oenococcus oeni|
|Citation:||International Journal of Food Microbiology, 2005; 105(2):233-244|
|Publisher:||Elsevier Science BV|
|Antonio Grimaldi, Eveline Bartowsky and Vladimir Jiranek|
|Abstract:||Lactic acid bacteria play an important role in winemaking by undertaking the malolactic fermentation, yet little information is available on other aspects of their physiology, such as their profile of external enzymatic activities. In this study we sought evidence for the existence and action of glycosidase enzymes in wine isolates of Oenococcus oeni. This group of enzymes is of interest because of their potential for liberation of grape-derived aroma compounds from their natural glycosylated state. This comprehensive study reveals that these bacteria produce glycosidases that might be important in winemaking. Strains did not necessarily hydrolyse all substrates tested, but rather were grouped according to substrate specificity. Thus a subset comprising strains 2, 5 and 16 possessed high cumulative activities against β-d- and α-d-glucopyranoside substrates, while a group comprising strains 4, 21 and 22 was noted for superior hydrolysis of β-d-xylopyranoside, α-l-rhamnopyranoside and α-l-arabinofuranoside substrates. Key physico-chemical inhibitors of analogous systems from other microorganisms were seen to produce variable responses across the strains investigated here. Accordingly, several strains retained significant hydrolytic activity at typical wine pH values (~3.0–4.0), residual glucose and fructose contents (up to 20 g/L), and ethanol contents (up to 12%). These findings highlight the potential of O. oeni as a useful alternative source of glycosidase enzymes for use in winemaking.|
|Keywords:||Glycosidase; Lactic acid bacteria; Wine; MLF; Aroma; Flavour|
|Description:||Copyright © 2005 Elsevier B.V. All rights reserved.|
|Appears in Collections:||Agriculture, Food and Wine publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.