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Type: Journal article
Title: A NAC domain protein interacts with tomato leaf curl virus replication accessory protein and enhances viral replication
Author: Selth, L.
Dogra, S.
Rasheed, M.
Healy, H.
Randles, J.
Rezaian, M.
Citation: The Plant Cell, 2005; 17(1):311-325
Publisher: Amer Soc Plant Physiologists
Issue Date: 2005
ISSN: 1040-4651
Statement of
Luke A. Selth, Satish C. Dogra, M. Saif Rasheed, Helen Healy, John W. Randles and M. Ali Rezaian
Abstract: Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SlNAC1, a new member of the NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SlNAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV REn as bait, and confirmed the interaction between these proteins in vitro. TLCV induces SlNAC1 expression specifically in infected cells, and this upregulation requires REn. In a transient TLCV replication system, overexpression of SlNAC1 resulted in a substantial increase in viral DNA accumulation. SlNAC1 colocalized with REn to the nucleus and activated transcription of a reporter gene in yeast, suggesting that in healthy cells it functions as a transcription factor. Together, these results imply that SlNAC1 plays an important role in the process by which REn enhances TLCV replication.
Keywords: Cell Nucleus
Plant Viruses
Lycopersicon esculentum
Plant Proteins
Transcription Factors
Viral Proteins
DNA, Viral
Evolution, Molecular
Virus Replication
Plant Diseases
Gene Expression Regulation, Plant
Gene Expression Regulation, Viral
Amino Acid Sequence
Base Sequence
Protein Structure, Tertiary
Molecular Sequence Data
Transcriptional Activation
Description: © 2004 American Society of Plant Biologists
DOI: 10.1105/tpc.104.027235
Appears in Collections:Agriculture, Food and Wine publications
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