Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/16995
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Type: Journal article
Title: A NAC domain protein interacts with tomato leaf curl virus replication accessory protein and enhances viral replication
Author: Selth, L.
Dogra, S.
Rasheed, M.
Healy, H.
Randles, J.
Rezaian, M.
Citation: The Plant Cell, 2005; 17(1):311-325
Publisher: Amer Soc Plant Physiologists
Issue Date: 2005
ISSN: 1040-4651
1532-298X
Statement of
Responsibility: 
Luke A. Selth, Satish C. Dogra, M. Saif Rasheed, Helen Healy, John W. Randles and M. Ali Rezaian
Abstract: Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SlNAC1, a new member of the NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SlNAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV REn as bait, and confirmed the interaction between these proteins in vitro. TLCV induces SlNAC1 expression specifically in infected cells, and this upregulation requires REn. In a transient TLCV replication system, overexpression of SlNAC1 resulted in a substantial increase in viral DNA accumulation. SlNAC1 colocalized with REn to the nucleus and activated transcription of a reporter gene in yeast, suggesting that in healthy cells it functions as a transcription factor. Together, these results imply that SlNAC1 plays an important role in the process by which REn enhances TLCV replication.
Keywords: Cell Nucleus
Plant Viruses
Lycopersicon esculentum
Plant Proteins
Transcription Factors
Viral Proteins
DNA, Viral
Evolution, Molecular
Phylogeny
Virus Replication
Plant Diseases
Gene Expression Regulation, Plant
Gene Expression Regulation, Viral
Up-Regulation
Amino Acid Sequence
Base Sequence
Protein Structure, Tertiary
Molecular Sequence Data
Transcriptional Activation
Description: © 2004 American Society of Plant Biologists
DOI: 10.1105/tpc.104.027235
Appears in Collections:Agriculture, Food and Wine publications
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