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Type: Journal article
Title: Severity-dependent expression of pro-inflammatory cytokines in traumatic spinal cord injury in the rat
Author: Yang, L.
Jones, N.
Blumbergs, P.
Van Den Heuvel, C.
Bradford, E.
Manavis, J.
Sarvestani, G.
Ghabriel, M.
Citation: Journal of Clinical Neuroscience, 2005; 12(3):276-284
Publisher: Churchill Livingstone
Issue Date: 2005
ISSN: 0967-5868
Statement of
Liqun Yang, Nigel R. Jones, Peter C. Blumbergs, Corinna Van Den Heuvel, Emma J. Moore, Jim Manavis, Ghafar T. Sarvestani and Mounir N. Ghabriel
Abstract: The post-traumatic inflammatory response in acute spinal cord contusion injury was studied in the rat. Mild and severe spinal cord injury (SCI) was produced by dropping a 10 g weight from 3 and 12 cm at the T12 vertebral level. Increased immunoreactivity of TNF-alpha in mild and severe SCI was detected in neurons at 1 h post-injury, and in neurons and microglia at 6 h post-injury, with a less significant increase in mild SCI. Expression was short-lived and declined sharply by 1 d post-injury. RT-PCR showed an early significant up-regulation of IL-1 beta, IL-6 and TNF-alpha mRNAs, maximal at 6 h post-injury with return to control levels by 24 h post-injury, the changes being less statistically significantly in mild SCI. Western blot showed early transient increases of IL-1 beta, IL-6 and TNF-alpha proteins in severe SCI but not mild SCI. Immunocytochemical, western blotting and RT-PCR analyses suggest that endogenous cells (neurons and microglia) in the spinal cord, not blood-borne leucocytes, contribute to IL-1 beta, IL-6 and TNF-alpha production in the post-traumatic inflammatory response and that their up-regulation is greater in severe than mild SCI.
Keywords: interleukin-1β
tumour necrosis factor-*
traumatic spinal cord injury
inflammatory response
Description: Copyright © 2005 Elsevier Ltd All rights reserved.
DOI: 10.1016/j.jocn.2004.06.011
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Appears in Collections:Anatomical Sciences publications
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