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Type: Journal article
Title: Population and virulence factor dynamics in fecal Escherichia coli from healthy adults consuming weight control diets
Author: McOrist, A.
Veuilett, G.
Vuaran, M.
Bird, A.
Noakes, M.
Topping, D.
Citation: Canadian Journal of Microbiology, 2005; 51(6):467-475
Publisher: Natl Research Council Canada
Issue Date: 2005
ISSN: 0008-4166
Statement of
A. McOrist, G. Veuilett, M. Vuaran, A. Bird, M. Noakes, and D. Topping
Abstract: Individual susceptibility to gastrointestinal infection is seen commonly in food poisoning outbreaks, but factors (such as diet) which may modulate this variability are understood poorly. Similarly, factors altering the population dynamics of enteric non-pathogenic Escherichia coli or of pathogenic E. coli containing toxin-signature DNA sequences in the colonic flora of healthy individuals are largely unknown. Feces were collected 4 times over a 12 week period from 41 healthy volunteer adults on a weight control diet (high or low in fiber). E. coli strains were examined by conventional culture followed by PCR for virulence genes stx1, stx2, eae and hlyA, and polymorphic beta-glucuronidase. Total E. coli counts ranged from undetectable to 8.75 log10 CFU/g feces and were unaffected by dietary fiber consumption or gender. Total E. coli counts were correlated positively with age (r = 0.401, P < 0.05). Fifty-eight percent (n = 24) of study individuals harboured more than 1 morph of beta-glucuronidase, indicating the presence of more than 1 strain of E. coli. Virulence genes were detected in 12 of 41 adults, comprising 10 stx1, 3 stx2, 3 eae, and 0 hlyA, but occurrence was not associated with diet, gender, or age. Factors influencing strain mobility over time did not appear to include diet or gender, while the positive relationship between total E. coli numbers and increasing age suggests that some older individuals are "more permissive" to mobile E. coli, including those with toxin genes.
Keywords: Feces
Escherichia coli
Escherichia coli Infections
Weight Loss
Adhesins, Bacterial
Escherichia coli Proteins
Colony Count, Microbial
Age Factors
Species Specificity
Genes, Bacterial
Dietary Fiber
Middle Aged
Hemolysin Proteins
Shiga Toxin 1
Shiga Toxin 2
Rights: Copyright © 2005 NRC Canada
DOI: 10.1139/W05-021
Appears in Collections:Aurora harvest 6
Obstetrics and Gynaecology publications

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