Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/17332
Citations
Scopus Web of ScienceĀ® Altmetric
?
?
Type: Journal article
Title: Up-regulation of Interleukin-8, Interleukin-10, Monocyte Chemotactic Protein-1, and Monocyte Chemotactic Protein-3 in Peripheral Blood Monocytes in Stable Lung Transplant Recipients: Are Immunosuppression Regimens Working?
Author: Hodge, G.
Hodge, S.
Reynolds, P.
Holmes, M.
Citation: Transplantation, 2005; 79(4):387-391
Publisher: Lippincott Williams & Wilkins
Issue Date: 2005
ISSN: 0041-1337
1534-6080
Abstract: <h4>Background</h4>Alveolar macrophages are a major source of inflammatory cytokines and chemokines involved in the pathogenesis of lung transplant rejection and are derived from blood monocytes that migrate to the lung. Levels of monocyte cytokines and chemokines that may be relevant in transplant rejection have not previously been determined in transplant recipients. We hypothesized that production of these inflammatory mediators by blood monocytes may be up-regulated despite the use of potent immunosuppression therapy.<h4>Method</h4>To investigate this, whole blood from 9 stable lung transplant recipients and 12 control volunteers was stimulated with lipopolysaccharide in vitro, and intracellular chemokine and cytokine production were determined with multiparameter flow cytometry.<h4>Results</h4>Monocyte production of chemokines interleukin (IL)-8, monocyte chemotactic protein (MCP)-1, and MCP-3, and anti-inflammatory cytokine IL-10 were significantly increased in the lung transplant group, but IL-6, tumor necrosis factor-alpha, IL-1alpha, IL-12, macrophage inflammatory protein-1alpha, macrophage inflammatory protein-1beta, and transforming growth factor-beta levels were unchanged.<h4>Conclusions</h4>Because MCP-3 is a major chemoattractant for leukocytes to sites of antigenic challenge and is a natural ligand for MCP-1 receptor, this novel finding has important implications for the pathogenesis of lung transplant rejection. We now provide evidence that current immunosuppression protocols have a limited effect on monocyte inflammatory cytokine production and do not adequately suppress monocyte IL-8, MCP-1, and MCP-3 chemokine production. Drugs that modulate the action of these chemokines may improve current protocols for reducing graft rejection. Intracellular chemokine and cytokine analysis with flow cytometry may be a more accurate indicator of immunosuppression than drug levels in these patients.
Keywords: Monocytes
Humans
Interleukin-8
Monocyte Chemoattractant Proteins
Interleukin-10
Cytokines
Immunosuppression
Lung Transplantation
Up-Regulation
Adult
Middle Aged
Chemokine CCL2
Chemokine CCL7
DOI: 10.1097/01.TP.0000151631.66884.2E
Appears in Collections:Aurora harvest 6
Paediatrics publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.