Please use this identifier to cite or link to this item:
Scopus Web of ScienceĀ® Altmetric
Type: Journal article
Title: Survival and engraftment of mouse embryonic stem cell-derived implants in the guinea pig brain
Author: Robinson, A.
Meedeniya, A.
Hemsley, K.
Auclair, D.
Crawley, A.
Hopwood, J.
Citation: Neuroscience Research, 2005; 53(2):161-168
Publisher: Elsevier Sci Ireland Ltd
Issue Date: 2005
ISSN: 0168-0102
Abstract: alpha-Mannosidosis is a lysosomal storage disease resulting from a deficiency of the enzyme alpha-D-mannosidase. A major feature of alpha-mannosidosis is progressive neurological decline, for which there is no safe and effective treatment available. We have a guinea pig model of alpha-mannosidosis that models the human condition. This study investigates the feasibility of implanting differentiated mouse embryonic stem cells in the neonatal guinea pig brain in order to provide a source of alpha-mannosidase to the affected central nervous system. Cells implanted at a low dose (1.5 x 10(3)cells per hemisphere) at 1 week of age were found to survive in very low numbers in some immunosuppressed animals out to 8 weeks. Four weeks post-implantation, cells implanted in high numbers (10(5) cells per hemisphere) formed teratomas in the majority of the animals implanted. Although implanted cells were found to migrate extensively within the brain and differentiate into mature cells of neural (and other) lineages, the safety issue related to uncontrolled cell proliferation precluded the use of this cell type for longer-term implantation studies. We conclude that the pluripotent cell type used in this study is unsuitable for achieving safe engraftment in the guinea pig brain.
Keywords: Brain
Multipotent Stem Cells
Guinea Pigs
Disease Models, Animal
Microscopy, Confocal
Stem Cell Transplantation
Cell Differentiation
Cell Movement
Graft Survival
DOI: 10.1016/j.neures.2005.06.010
Appears in Collections:Aurora harvest 2
Paediatrics publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.