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Type: Journal article
Title: The HLDA8 blind panel: findings and conclusions
Author: Swart, B.
Salganik, M.
Wand, M.
Tinckam, K.
Milford, E.
Drbal, K.
Angelisova, P.
Horejsi, V.
Macardle, P.
Bailey, S.
Hollemweguer, E.
Hodge, G.
Nairn, J.
Millard, D.
Dagdeviren, A.
Dandie, G.
Zola, H.
Citation: Journal of Immunological Methods, 2005; 305(1):75-83
Publisher: Elsevier Science BV
Issue Date: 2005
ISSN: 0022-1759
Statement of
Bernadette Swart, Mikhail P. Salganik, Matthew P. Wand, Kathryn Tinckam, Edgar L. Milford, Karel Drbal, Pavla Angelisova, Vaclav Horejsi, Peter Macardle, Sheree Bailey, Enoc Hollemweguer, Greg Hodge, Judith Nairn, Debbrah Millard, Attila Dagdeviren, Geoffrey W. Dandie, Heddy Zola
Abstract: There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity.
Keywords: HLDA8 blind panel; Cell Line; Humans; Antigens, CD; Antibodies; Antigens, Surface; Blotting, Western; Immunochemistry; Antibody Specificity
Rights: © 2005 Elsevier B.V. All rights reserved
RMID: 0020051174
DOI: 10.1016/j.jim.2005.07.003
Appears in Collections:Paediatrics publications

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