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dc.contributor.authorSwart, B.en
dc.contributor.authorSalganik, M.en
dc.contributor.authorWand, M.en
dc.contributor.authorTinckam, K.en
dc.contributor.authorMilford, E.en
dc.contributor.authorDrbal, K.en
dc.contributor.authorAngelisova, P.en
dc.contributor.authorHorejsi, V.en
dc.contributor.authorMacardle, P.en
dc.contributor.authorBailey, S.en
dc.contributor.authorHollemweguer, E.en
dc.contributor.authorHodge, G.en
dc.contributor.authorNairn, J.en
dc.contributor.authorMillard, D.en
dc.contributor.authorDagdeviren, A.en
dc.contributor.authorDandie, G.en
dc.contributor.authorZola, H.en
dc.identifier.citationJournal of Immunological Methods, 2005; 305(1):75-83en
dc.description.abstractThere were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity.en
dc.description.statementofresponsibilityBernadette Swart, Mikhail P. Salganik, Matthew P. Wand, Kathryn Tinckam, Edgar L. Milford, Karel Drbal, Pavla Angelisova, Vaclav Horejsi, Peter Macardle, Sheree Bailey, Enoc Hollemweguer, Greg Hodge, Judith Nairn, Debbrah Millard, Attila Dagdeviren, Geoffrey W. Dandie, Heddy Zolaen
dc.publisherElsevier Science BVen
dc.rights© 2005 Elsevier B.V. All rights reserveden
dc.subjectHLDA8 blind panel; Cell Line; Humans; Antigens, CD; Antibodies; Antigens, Surface; Blotting, Western; Immunochemistry; Antibody Specificityen
dc.titleThe HLDA8 blind panel: findings and conclusionsen
dc.typeJournal articleen
pubs.library.collectionPaediatrics publicationsen
Appears in Collections:Paediatrics publications

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