Please use this identifier to cite or link to this item:
Scopus Web of ScienceĀ® Altmetric
Type: Journal article
Title: Activation of the sphingosine kinase-signaling pathway by high glucose mediates the proinflammatory phenotype of endothelial cells
Author: Wang, L.
Xing, X.
Holmes, A.
Wadham, C.
Gamble, J.
Vadas, M.
Xia, P.
Citation: Circulation Research, 2005; 97(9):891-899
Publisher: Lippincott Williams & Wilkins
Issue Date: 2005
ISSN: 0009-7330
Statement of
Lijun Wang,Xiao-Ping Xing,Andrew Holmes,Carol Wadham,Jennifer R. Gamble,Mathew A. Vadas,Pu Xia
Abstract: Vascular endothelial cells are key targets for hyperglycemic damage that facilitates vascular inflammation and the vasculopathy associated with diabetes mellitus. However, the mechanisms underlying this damage remain undefined. We now demonstrate that hyperglycemia induces activation of sphingosine kinase (SphK), which represents a novel signaling pathway that mediates endothelial damage under ambient high glucose conditions. SphK activity was significantly increased in aorta and heart of streptozotocin-induced diabetic rats. Interestingly, this increase in SphK activity was prevented by insulin treatment, which achieved euglycemia in the diabetic animals. Hyperglycemia-induced increase in SphK activity was also evident in endothelial cells that received long-term exposure to high glucose (22 mmol/L). Studies using a small interfering RNA strategy demonstrated that endogenous SphK1, but not SphK2, is the major isoenzyme that was activated by high glucose. In addition, an increase in SphK1 phosphorylation was detected in a protein kinase C- and extracellular signal-regulated kinase 1/2-dependent manner, which accounts for the high glucose-induced increases in SphK activity. Importantly, inhibition of SphK1 by either a chemical inhibitor (N',N'-dimethylsphingosine) or expression of a dominant-negative mutant of SphK1 (SphK(G82D)), or SphK1-specific small interfering RNA, strongly protected endothelial cells against high glucose-induced damage, as characterized by an attenuation in the expression of proinflammatory adhesion molecules, adhesion of leukocytes to endothelial cells, and nuclear factor kappaB activation. Thus, interventions that target the SphK-signaling pathway may have the potential to prevent vascular lesions under hyperglycemic conditions.
Keywords: Cells, Cultured
Endothelial Cells
Rats, Sprague-Dawley
Diabetic Angiopathies
Diabetes Mellitus, Experimental
Pertussis Toxin
Phosphotransferases (Alcohol Group Acceptor)
Extracellular Signal-Regulated MAP Kinases
Protein Kinase C
NF-kappa B
Receptors, Lysosphingolipid
Signal Transduction
Enzyme Activation
DOI: 10.1161/01.RES.0000187469.82595.15
Published version:
Appears in Collections:Aurora harvest 2
Medicine publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.