Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/17495
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Type: Journal article
Title: The opsonising activity of a pentraxin-like protein isolated from snapper (Pagrus auratus, Sparidae) serum
Author: Cook, M.
Hayball, P.
Nowak, B.
Hayball, J.
Citation: Developmental and Comparative Immunology, 2005; 29(8):703-712
Publisher: Pergamon-Elsevier Science Ltd
Issue Date: 2005
ISSN: 0145-305X
1879-0089
Statement of
Responsibility: 
Mathew T. Cook, Peter J. Hayball, Barbara F. Nowak, John D. Hayball
Abstract: In mammals the pentraxins, C-reactive protein (CRP) and serum amyloid P component (SAP) are important components of the immune response. Although pentraxins have been isolated from a number of fish species few studies detail their functional immunological role. In this paper we report the establishment of a flow cytometry based assay to measure the phagocytic activity of isolated snapper head kidney leukocytes (HKLs). This assay was then used to examine the ability of a pentraxin-like protein isolated from the serum of snapper (P. auratus) (Sn-PLP) to act as an opsonin. Incubation of snapper head kidney leukocytes (HKL) with FITC-labelled beads resulted in uptake of these particles by approximately 35% of HKLs. Incubation of beads with Sn-PLP enhanced phagocytosis by snapper HKLs in a dose-dependant manner. Enhanced phagocytosis could be inhibited by addition of a rabbit anti-Sn-PLP antibody suggesting that Sn-PLP may act as a ligand for a HKL cell surface receptor. This study provides further evidence toward a functional role for pentraxins in the host defence repertoire of fish.
Keywords: Head
Kidney
Leukocytes
Animals
Fishes
Azides
Fluorescein-5-isothiocyanate
C-Reactive Protein
Serum Amyloid P-Component
Fish Proteins
Flow Cytometry
Phagocytosis
Opsonin Proteins
DOI: 10.1016/j.dci.2004.05.009
Appears in Collections:Aurora harvest 2
Medicine publications

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