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https://hdl.handle.net/2440/17495
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dc.contributor.author | Cook, M. | - |
dc.contributor.author | Hayball, P. | - |
dc.contributor.author | Nowak, B. | - |
dc.contributor.author | Hayball, J. | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | Developmental and Comparative Immunology, 2005; 29(8):703-712 | - |
dc.identifier.issn | 0145-305X | - |
dc.identifier.issn | 1879-0089 | - |
dc.identifier.uri | http://hdl.handle.net/2440/17495 | - |
dc.description.abstract | In mammals the pentraxins, C-reactive protein (CRP) and serum amyloid P component (SAP) are important components of the immune response. Although pentraxins have been isolated from a number of fish species few studies detail their functional immunological role. In this paper we report the establishment of a flow cytometry based assay to measure the phagocytic activity of isolated snapper head kidney leukocytes (HKLs). This assay was then used to examine the ability of a pentraxin-like protein isolated from the serum of snapper (P. auratus) (Sn-PLP) to act as an opsonin. Incubation of snapper head kidney leukocytes (HKL) with FITC-labelled beads resulted in uptake of these particles by approximately 35% of HKLs. Incubation of beads with Sn-PLP enhanced phagocytosis by snapper HKLs in a dose-dependant manner. Enhanced phagocytosis could be inhibited by addition of a rabbit anti-Sn-PLP antibody suggesting that Sn-PLP may act as a ligand for a HKL cell surface receptor. This study provides further evidence toward a functional role for pentraxins in the host defence repertoire of fish. | - |
dc.description.statementofresponsibility | Mathew T. Cook, Peter J. Hayball, Barbara F. Nowak, John D. Hayball | - |
dc.language.iso | en | - |
dc.publisher | Pergamon-Elsevier Science Ltd | - |
dc.source.uri | http://dx.doi.org/10.1016/j.dci.2004.05.009 | - |
dc.subject | Head | - |
dc.subject | Kidney | - |
dc.subject | Leukocytes | - |
dc.subject | Animals | - |
dc.subject | Fishes | - |
dc.subject | Azides | - |
dc.subject | Fluorescein-5-isothiocyanate | - |
dc.subject | C-Reactive Protein | - |
dc.subject | Serum Amyloid P-Component | - |
dc.subject | Fish Proteins | - |
dc.subject | Flow Cytometry | - |
dc.subject | Phagocytosis | - |
dc.subject | Opsonin Proteins | - |
dc.title | The opsonising activity of a pentraxin-like protein isolated from snapper (Pagrus auratus, Sparidae) serum | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.dci.2004.05.009 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Hayball, J. [0000-0002-3089-4506] | - |
Appears in Collections: | Aurora harvest 2 Medicine publications |
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