Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/17495
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dc.contributor.authorCook, M.-
dc.contributor.authorHayball, P.-
dc.contributor.authorNowak, B.-
dc.contributor.authorHayball, J.-
dc.date.issued2005-
dc.identifier.citationDevelopmental and Comparative Immunology, 2005; 29(8):703-712-
dc.identifier.issn0145-305X-
dc.identifier.issn1879-0089-
dc.identifier.urihttp://hdl.handle.net/2440/17495-
dc.description.abstractIn mammals the pentraxins, C-reactive protein (CRP) and serum amyloid P component (SAP) are important components of the immune response. Although pentraxins have been isolated from a number of fish species few studies detail their functional immunological role. In this paper we report the establishment of a flow cytometry based assay to measure the phagocytic activity of isolated snapper head kidney leukocytes (HKLs). This assay was then used to examine the ability of a pentraxin-like protein isolated from the serum of snapper (P. auratus) (Sn-PLP) to act as an opsonin. Incubation of snapper head kidney leukocytes (HKL) with FITC-labelled beads resulted in uptake of these particles by approximately 35% of HKLs. Incubation of beads with Sn-PLP enhanced phagocytosis by snapper HKLs in a dose-dependant manner. Enhanced phagocytosis could be inhibited by addition of a rabbit anti-Sn-PLP antibody suggesting that Sn-PLP may act as a ligand for a HKL cell surface receptor. This study provides further evidence toward a functional role for pentraxins in the host defence repertoire of fish.-
dc.description.statementofresponsibilityMathew T. Cook, Peter J. Hayball, Barbara F. Nowak, John D. Hayball-
dc.language.isoen-
dc.publisherPergamon-Elsevier Science Ltd-
dc.subjectHead-
dc.subjectKidney-
dc.subjectLeukocytes-
dc.subjectAnimals-
dc.subjectFishes-
dc.subjectAzides-
dc.subjectFluorescein-5-isothiocyanate-
dc.subjectC-Reactive Protein-
dc.subjectSerum Amyloid P-Component-
dc.subjectFish Proteins-
dc.subjectFlow Cytometry-
dc.subjectPhagocytosis-
dc.subjectOpsonin Proteins-
dc.titleThe opsonising activity of a pentraxin-like protein isolated from snapper (Pagrus auratus, Sparidae) serum-
dc.typeJournal article-
dc.identifier.doi10.1016/j.dci.2004.05.009-
pubs.publication-statusPublished-
dc.identifier.orcidHayball, J. [0000-0002-3089-4506]-
Appears in Collections:Aurora harvest 2
Medicine publications

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