Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/17496
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Type: Journal article
Title: Assessment of snapper (Pagrus auratus) natural IgM binding to bromelain treated sheep erythrocytes
Author: Morrison, R.
Lyons, A.
Nowak, B.
Hayball, J.
Citation: Fish & Shellfish Immunology, 2005; 18(1):91-99
Publisher: Academic Press Ltd
Issue Date: 2005
ISSN: 1050-4648
1095-9947
Statement of
Responsibility: 
Richard N. Morrison, A. Bruce Lyons, Barbara F. Nowak, John D. Hayball
Abstract: Normal snapper (Pagrus auratus Bloch and Schneider) serum was examined for natural IgM that binds to protease (bromelain) treated sheep erythrocytes (BrSRBC) in a model assay system that has been used to appraise natural IgM of various mammals. Normal snapper serum lysed BrSRBC while haemolysis was abrogated by heat inactivation of serum and in divalent cation-deficient conditions, indicative of classical complement mediated lysis. In addition, heat inactivated normal snapper serum agglutinated BrSRBC while phosphatidylcholine (PtC) liposomes partially inhibited both haemolysis and agglutination. Inhibition of haemolysis and agglutination may have been mediated by an interaction between immunoglobulin (Ig) and PtC as protein A purified snapper Ig bound to PtC liposomes. However it is not known if this binding was PtC specific nor if the binding was initiated by either the Fab and/or Fc domains of snapper Ig. BrSRBC plaque forming cells (PFC) were detected in the peritoneal cavity, spleen, head kidney and peripheral blood of normal snapper. The greatest proportion of BrSRBC PFC per B cell was within the peritoneal cavity followed by the spleen, peripheral blood and head kidney. Together, these data suggest that normal snapper serum may contain natural Ig that binds BrSRBC, activating the classical complement cascade.
Keywords: Natural immunoglobulin; Complement; Erythrocytes; Bromelain; Snapper
Rights: Copyright © 2004 Elsevier Ltd. All rights reserved.
RMID: 0020052242
DOI: 10.1016/j.fsi.2004.06.006
Appears in Collections:Medicine publications

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