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|Title:||Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)|
|Citation:||Journal of Virological Methods, 2003; 114(1):65-69|
|Publisher:||Elsevier Science BV|
|Genet Mekuria, Sunita A. Ramesh, Evita Alberts, Terry Bertozzi, Michelle Wirthensohn, Graham Collins and Margaret Sedgley|
|Abstract:||A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.|
|Keywords:||Ilarvirus; Prune dwarf virus; ELISA; Prunus necrotic ringspot virus|
|Rights:||Copyright © 2003 Elsevier B.V. All rights reserved.|
|Appears in Collections:||Environment Institute publications|
Agriculture, Food and Wine publications
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