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Type: Journal article
Title: Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)
Author: Mekuria, G.
Ramesh, S.
Alberts, E.
Bertozzi, T.
Wirthensohn, M.
Collins, G.
Sedgley, M.
Citation: Journal of Virological Methods, 2003; 114(1):65-69
Publisher: Elsevier Science BV
Issue Date: 2003
ISSN: 0166-0934
Statement of
Genet Mekuria, Sunita A. Ramesh, Evita Alberts, Terry Bertozzi, Michelle Wirthensohn, Graham Collins and Margaret Sedgley
Abstract: A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.
Keywords: Ilarvirus; Prune dwarf virus; ELISA; Prunus necrotic ringspot virus
Rights: Copyright © 2003 Elsevier B.V. All rights reserved.
RMID: 0020031046
DOI: 10.1016/j.jviromet.2003.08.014
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Appears in Collections:Environment Institute publications
Agriculture, Food and Wine publications

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