Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/18928
Type: Thesis
Title: Characterisation of a tannin acylhydrolase from a ruminal selenomonad / by Ian Skene.
Author: Skene, Ian
Issue Date: 1996
School/Discipline: Dept. of Animal Sciences
Abstract: The aim of this PhD project is to screen feral goat rumen fluid for the presence of new organisms that may play a role in the detoxification of tannins and to investigate their mechanisms of action. An enrichment experiment is conducted to screen rumen fluid for anaerobic bacteria capable of growing in the presence of high levels of "Acacia" condensed tannin. Four morphologically-distinct bacteria are isolated, confirming that resistance is a property shared by more than one organism. One isolate is chosen at random for further characterisation and is identified as a strain of "Selenomonas ruminantium" subspecies "ruminantium". It is arbitrarily designated strain K2. "Selenomonas ruminantium" K2 is shown to be not only tannin-resistant but also able to grow on tannic acid. It is proposed that this bacterium obtained energy for growth from tannic acid. The thesis examines the molecular mechanisms controlling tannin resistance or tannin degradation in rumen microorganisms.
Dissertation Note: Thesis (Ph.D.)--University of Adelaide, Dept. of Animal Science, 1997
Subject: Anaerobic bacteria.
Rumen Microbiology.
Molecular cloning.
Oligonucleotides.
Tannins.
Anaerobic bacteria Molecular aspects.
Description: Bibliography: leaves 189-205.
xi, 205 leaves : ill. ; 30 cm.
Provenance: This electronic version is made publicly available by the University of Adelaide in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. This thesis may incorporate third party material which has been used by the author pursuant to Fair Dealing exception. If you are the author of this thesis and do not wish it to be made publicly available or If you are the owner of any included third party copyright material you wish to be removed from this electronic version, please complete the take down form located at: http://www.adelaide.edu.au/legals
Appears in Collections:Research Theses

Files in This Item:
File Description SizeFormat 
01front.pdf575.22 kBAdobe PDFView/Open
02whole.pdf10.09 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.