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|Title:||Targeted transgenesis and the 186 site-specific recombination system / by Sharon Jane Harrison.|
|Author:||Harrison, Sharon Jane|
|School/Discipline:||Dept. of Biochemistry|
|Abstract:||Characterises the 186 coliphage site-specific integration reaction in vitro with the expectation that it could be used in mammalian systems to insert genes or modify existing ones, to provide an alternative method for the production of transgenic livestock species. The analyzed system is the temperate bacteriophage 186. The in-vitro requirements for 186 integrative site-specific recombination were investigated. In vivo investigations were conducted whereby active 186-intasomes were microinjected into fertilised mouse eggs containing genomic copies of 186-attB. The 186 system has not been shown to work in vivo.|
|Dissertation Note:||Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry 1999?|
|Description:||Bibliography: leaves 120-138.|
xi, 138,  leaves : ill. ; 30 cm.
|Provenance:||This electronic version is made publicly available by the University of Adelaide in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. This thesis may incorporate third party material which has been used by the author pursuant to Fair Dealing exception. If you are the author of this thesis and do not wish it to be made publicly available or If you are the owner of any included third party copyright material you wish to be removed from this electronic version, please complete the take down form located at: http://www.adelaide.edu.au/legals|
|Appears in Collections:||Research Theses|
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|02whole.pdf||9.95 MB||Adobe PDF||View/Open|
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