Please use this identifier to cite or link to this item:
Type: Thesis
Title: Regulation of the human interleukin-3 gene / by Abbas Mohammed Hawwari.
Author: Hawwari, Abbas Mohammed
Issue Date: 2001
School/Discipline: Dept. of Medicine
Abstract: To understand the regulation of the human IL-3 gene, this study has investigated the functions of DNase I hypersensitive (DH) sites located upstream and downstream of the gene and focused on a prominent pair of sites located 4.5 kb and 4.1 kb upstream of the IL-3 gene. A 245 bp SacI/StuI DNA fragment (SS245) encompassing the -4.5 kb site increased the activity of a minimal promoter 20 fold in luciferase reporter gene plasmid upon transfection into Jurkat T cells and activation with a combination of phorbol ester (PMA) and calcium ionophore (CaI). In contrast, the -4.1 kb DH site did not increase the activity of the promoter in transient transfection assays, indicating an absence of enhancer activity.
Dissertation Note: Thesis (Ph.D.)--University of Adelaide, Dept. of Medicine, 2001?
Description: Bibliography: leaves 169-199.
x, 199 leaves, [69] leaves of plates : ill. (some col.) ; 30 cm.
Provenance: This electronic version is made publicly available by the University of Adelaide in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. This thesis may incorporate third party material which has been used by the author pursuant to Fair Dealing exception. If you are the author of this thesis and do not wish it to be made publicly available or If you are the owner of any included third party copyright material you wish to be removed from this electronic version, please complete the take down form located at:
Appears in Collections:Research Theses

Files in This Item:
File Description SizeFormat 
01front.pdf 81.36 kBAdobe PDFView/Open
02whole.pdf12.59 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.