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Type: Journal article
Title: Identification of incompatibility genotypes in almond (Prunus dulcis Mill.) using specific primers based on the introns of the S-alleles
Author: Channuntapipat, C.
Wirthensohn, M.
Ramesh, S.
Batlle, I.
Arus, P.
Sedgley, M.
Collins, G.
Citation: Plant Breeding, 2003; 122(2):164-168
Publisher: Blackwell Wissenschafts-verlag GMBH
Issue Date: 2003
ISSN: 0179-9541
Statement of
C. Channuntapipat, M. Wirthensohn, S.A. Ramesh, I. Batlle, P. Arús, M. Sedgley, G. Collins
Abstract: Identification of the incompatibility genotypes of almond cultivars is important in breeding programmes for designing crosses and for selecting progeny. This paper describes a novel molecular technique for the identification of S-alleles in almond based on the use of PCR primers designed from the sequences of the introns without the need for restriction enzyme digestion. Nine specific pairs of primers have been designed for the S1, S2, S5, S7, S8, S9, S10 (putative), S23 and Sf alleles, and these confirmed the S-allele specificities for 22 of the 23 accessions for which published information is available. This technique provides a precise method for identifying S-alleles from the genomic DNAs of almond cultivars, and will be useful for confirming the segregation of alleles in breeding progeny.
Keywords: Prunus dulcis; Self-incompatibility; S-allele; Molecular marker; PCR
RMID: 0020030249
DOI: 10.1046/j.1439-0523.2003.00842.x
Appears in Collections:Agriculture, Food and Wine publications

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