Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/23047
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | HIV type 1 persistence in CD4¯/CD8¯ double negative T cells from patients on antiretroviral therapy |
Other Titles: | HIV type 1 persistence in CD4-bar/CD8-bar double negative T cells from patients on antiretroviral therapy |
Author: | Cheney, K. Kumar, R. Purins, A. Mundy, L. Fergusson, A. Shaw, D. Burrell, C. Li, P. |
Citation: | AIDS Research and Human Retroviruses, 2006; 22(1):66-75 |
Publisher: | Mary Ann Liebert Inc Publ |
Issue Date: | 2006 |
ISSN: | 0889-2229 1931-8405 |
Statement of Responsibility: | Kelly M. Cheney, Raman Kumar, Adrian Purins, Linda Mundy, Wendy Ferguson, David Shaw, Christopher J. Burrell, and Peng LI. |
Abstract: | The establishment of reservoirs of latently infected cells is thought to contribute to the persistence of HIV-1 infection in the host. Studies so far have mainly focused on the long-lived reservoir of HIV-infected resting CD4+ T cells. A discrete population of HIV-infected CD4¯/CD8¯ double negative (DN) T cells has recently been shown to exist and may also play a role in HIV-1 persistence. DN T cells are CD3 positive, either TCRαβ or TCRγδ positive, but lack both CD4 and CD8 surface markers. We developed a novel, magnetic bead column-based cell fractionation procedure for isolating >99% pure DN T cells. CD4+, CD8+, and DN T cells were purified from 23 samples of a cohort of 18 HIV-1-infected patients. Each cell fraction was analyzed for levels of total and integrated HIV-1 DNA. A correlation was observed between the presence of HIV-1 DNA in the DN T cell fraction and plasma viral load (VL). Using a micrococulture technique, we saw an initial release of virus from DN T cells of a patient with high VL. Analysis of env and nef sequence data suggested that the HIV-1 present in CD4+ and DN T cells originated from a common infecting strain. Different from the published literature, we have demonstrated the presence of HIV-1 DNA in DN T cells only in patients who are experiencing HAART failure. While these cells may have a limited role in viral persistence in high VL patients, our results suggest DN T cells are unlikely to be a major reservoir in patients on HAART with clinically undetectable plasma viral RNA. |
Keywords: | T-Lymphocytes Humans HIV-1 HIV Infections RNA, Viral Anti-HIV Agents Antiretroviral Therapy, Highly Active Viral Load Adult Middle Aged Male CD4 Antigens CD8 Antigens |
Rights: | © Mary Ann Liebert, Inc |
DOI: | 10.1089/aid.2006.22.66 |
Published version: | http://www.liebertonline.com/doi/abs/10.1089/aid.2006.22.66 |
Appears in Collections: | Aurora harvest 2 Public Health publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.