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|Title:||HIV type 1 persistence in CD4¯/CD8¯ double negative T cells from patients on antiretroviral therapy|
|Other Titles:||HIV type 1 persistence in CD4-bar/CD8-bar double negative T cells from patients on antiretroviral therapy|
|Citation:||Aids Research and Human Retroviruses, 2006; 22(1):66-75|
|Publisher:||Mary Ann Liebert Inc Publ|
|Kelly M. Cheney, Raman Kumar, Adrian Purins, Linda Mundy, Wendy Ferguson, David Shaw, Christopher J. Burrell, and Peng LI.|
|Abstract:||The establishment of reservoirs of latently infected cells is thought to contribute to the persistence of HIV-1 infection in the host. Studies so far have mainly focused on the long-lived reservoir of HIV-infected resting CD4+ T cells. A discrete population of HIV-infected CD4¯/CD8¯ double negative (DN) T cells has recently been shown to exist and may also play a role in HIV-1 persistence. DN T cells are CD3 positive, either TCRαβ or TCRγδ positive, but lack both CD4 and CD8 surface markers. We developed a novel, magnetic bead column-based cell fractionation procedure for isolating >99% pure DN T cells. CD4+, CD8+, and DN T cells were purified from 23 samples of a cohort of 18 HIV-1-infected patients. Each cell fraction was analyzed for levels of total and integrated HIV-1 DNA. A correlation was observed between the presence of HIV-1 DNA in the DN T cell fraction and plasma viral load (VL). Using a micrococulture technique, we saw an initial release of virus from DN T cells of a patient with high VL. Analysis of env and nef sequence data suggested that the HIV-1 present in CD4+ and DN T cells originated from a common infecting strain. Different from the published literature, we have demonstrated the presence of HIV-1 DNA in DN T cells only in patients who are experiencing HAART failure. While these cells may have a limited role in viral persistence in high VL patients, our results suggest DN T cells are unlikely to be a major reservoir in patients on HAART with clinically undetectable plasma viral RNA.|
|Keywords:||T-Lymphocytes; Humans; HIV-1; HIV Infections; RNA, Viral; Anti-HIV Agents; Antiretroviral Therapy, Highly Active; Viral Load; Adult; Middle Aged; Male; CD4 Antigens; CD8 Antigens|
|Rights:||© Mary Ann Liebert, Inc|
|Appears in Collections:||Public Health publications|
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