Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/23194
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Type: Journal article
Title: Remodeling of extracellular matrix at ovulation of the bovine ovarian follicle
Author: Irving-Rodgers, H.
Catanzariti, K.
Aspden, W.
D'Occhio, M.
Rodgers, R.
Citation: Molecular Reproduction and Development, 2006; 73(10):1292-1302
Publisher: Wiley-Liss
Issue Date: 2006
ISSN: 1040-452X
1098-2795
Statement of
Responsibility: 
H.F. Irving-Rodgers, K.D. Catanzariti, W.J. Aspden, M.J. D'Occhio, R.J. Rodgers
Abstract: Using immunohistochemistry and RNA analyses we examined the fate of components of a newly identified matrix that develops between granulosa cells (focimatrix, abbreviated from focal intraepithelial matrix) and of the follicular basal lamina in ovulating bovine ovarian follicles. Pre- and postovulatory follicles were generated by treatment with estradiol (Day 1), progesterone (Days 1-10), and prostaglandin analogue (Day 9) with either no further treatment (Group 1, n = 6) and or with 25 mg porcine LH (Day 11, Group 2, n = 8 or Day 10, Group 3, n = 8) and ovariectomy on Day 12 (12-14 hr post LH in Group 2, 38-40.5 hr in Group 3). In the time frame examined no loss of follicular basal lamina laminin chains beta2 and gamma1 or nidogen 1 was observed. In the follicular basal lamina collagen type IV alpha1 and perlecan were present prior to ovulation; after ovulation collagen type IV alpha1 was discontinuously distributed and perlecan was absent. Versican in the theca interna adjacent to the follicular basal lamina in preovulatory follicles was not observed post ovulation, however, the granulosa cells then showed strong cytoplasmic staining for versican. Expression of versican isoforms V0, V1, and V3 was detected at all stages. Focimatrix was observed in preovulatory follicles. It contained collagen type IV alpha1, laminins beta2 and gamma1, nidogen 1 and perlecan and underwent changes in composition similar to that of the follicular basal lamina. In conclusion focimatrix and the follicular basal lamina are degraded at ovulation. Individual components are lost at different times.
Keywords: follicle; ovulation; basal lamina; focimatrix; extracellular matrix
Description: The definitive version may be found at www.wiley.com
Rights: Copyright © 2006 Wiley-Liss, Inc.
RMID: 0020061359
DOI: 10.1002/mrd.20580
Published version: http://www3.interscience.wiley.com/cgi-bin/abstract/112725995
Appears in Collections:Obstetrics and Gynaecology publications

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