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https://hdl.handle.net/2440/23195
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dc.contributor.author | Shavlakadze, T. | - |
dc.contributor.author | Boswell, J. | - |
dc.contributor.author | Burt, D. | - |
dc.contributor.author | Asante, E. | - |
dc.contributor.author | Tomas, F. | - |
dc.contributor.author | Davies, M. | - |
dc.contributor.author | White, J. | - |
dc.contributor.author | Grounds, M. | - |
dc.contributor.author | Goddard, C. | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | Growth Hormone and IGF Research, 2006; 16(3):157-173 | - |
dc.identifier.issn | 1096-6374 | - |
dc.identifier.issn | 1532-2238 | - |
dc.identifier.uri | http://hdl.handle.net/2440/23195 | - |
dc.description.abstract | Human IGF-I was over-expressed in skeletal muscles of C57/BL6xCBA mice under the control of the rat skeletal alpha-actin gene promoter. RT-PCR verified expression of the transgene in skeletal muscle but not in the liver of 1- and 21-day old heterozygote transgenic mice. The concentration of endogenous mouse IGF-I, measured by an immunoassay which does not detect human IGF-I, was not significantly different between transgenic mice and wild-type littermates (9.5 +/- 0.8 and 13.3 +/- 1.9 ng/g in muscle; 158.3 +/- 18.6 and 132.9 +/- 33.1 ng/ml in plasma, respectively). In contrast, quantitation with antibodies to human IGF-I showed an increase in IGF-I of about 100 ng/ml in plasma and 150 ng/g in muscle of transgenic mice at 6 months of age. Transgenic males, compared to their age matched wild-type littermates, had a significantly higher body weight (38.6 +/- 0.53 g vs. 35.8 +/- 0.64 g at 6 months of age; P < 0.001), dry fat-free carcass mass (5.51 +/- 0.085 vs. 5.08 +/- 0.092 g; P < 0.001) and myofibrillar protein mass (1.62 +/- 0.045 vs. 1.49 +/- 0.048 g; P < 0.05), although the fractional content of fat in the carcass was lower (167 +/- 7.0 vs. 197 +/- 7.7 g/kg wet weight) in transgenic animals. There was no evidence of muscle hypertrophy and no change in the proportion of slow type I myofibres in the limb muscles of Rskalpha-actin/hIGF-I transgenic mice at 3 or 6 months of age. Phenotypic changes in Rskalpha-actin/hIGF-I mice are likely to be due to systemic as well as autocrine/paracrine effects of overproduction of IGF-I due to expression of the human IGF-I transgene. The effect of muscle specific over-expression of Rskalpha-actin/hIGF-I transgene was tested on: (i) muscle regeneration in auto-transplanted whole muscle grafts; (ii) myofibre atrophy following sciatic nerve transection; and (iii) sarolemmal damage and myofibre necrosis in dystrophic mdx muscle. No beneficial effect of muscle specific over-expression of Rskalpha-actin/hIGF-I transgene was seen in these three experimental models. | - |
dc.description.statementofresponsibility | T. Shavlakadze, J.M. Boswell, D.W. Burt, E.A. Asante, F.M. Tomas, M.J. Davies, J.D. White, M.D. Grounds, C. Goddard | - |
dc.language.iso | en | - |
dc.publisher | Churchill Livingstone | - |
dc.rights | © 2006, Elsevier | - |
dc.source.uri | http://dx.doi.org/10.1016/j.ghir.2005.11.003 | - |
dc.subject | IGF-1 | - |
dc.subject | Transgenic mice | - |
dc.subject | skeletal muscle | - |
dc.subject | circulating IGF-1 | - |
dc.subject | growth | - |
dc.subject | regeneration | - |
dc.subject | atrophy | - |
dc.subject | mdx | - |
dc.title | Rskα-actin/hIGF-1 transgenic mice with increased IGF-I in skeletal muscle and blood: Impact on regeneration, denervation and muscular dystrophy | - |
dc.title.alternative | Rsk alpha-actin/hIGF-1 transgenic mice with increased IGF-I in skeletal muscle and blood: Impact on regeneration, denervation and muscular dystrophy | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.ghir.2005.11.003 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 2 Obstetrics and Gynaecology publications |
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