Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/23286
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Type: Journal article
Title: Enzyme replacement therapy in alpha-mannosidosis guinea-pigs
Author: Crawley, A.
King, B.
Berg, T.
Meikle, P.
Hopwood, J.
Citation: Molecular Genetics and Metabolism, 2006; 89(1-2):48-57
Publisher: Academic Press Inc Elsevier Science
Issue Date: 2006
ISSN: 1096-7192
1096-7206
Statement of
Responsibility: 
Allison C. Crawley, Barbara King, Thomas Berg, a, Peter J. Meikle and John J. Hopwood
Abstract: alpha-Mannosidosis is a lysosomal storage disorder caused by deficient activity of lysosomal alpha-mannosidase and is characterised by massive accumulation of mannose-containing oligosaccharides in affected individuals. Patients develop behaviour and learning difficulties, skeletal abnormalities, immune deficiency and hearing impairment. Disease in alpha-mannosidosis guinea-pigs resembles the clinical, histopathological, biochemical and molecular features of the human disease. We have used the guinea-pig model to investigate efficacy of enzyme replacement therapy as a treatment for alpha-mannosidosis. Intravenous recombinant human lysosomal alpha-mannosidase, administered at a dose of 1mg/kg, was cleared from circulation with a half-life of 53 h, with significant enzyme activity (1.4x normal levels) detected in circulation one week post-injection. alpha-Mannosidase administered to alpha-mannosidosis guinea-pigs at 1mg/kg (onset at birth or approximately 30 days) and 10mg/kg (at birth) was distributed widely amongst tissues, including to capillary depleted brain. By monitoring with tandem mass spectrometry, enzyme replacement therapy was found to be effective in reducing stored substrates in peripheral tissues at both dose rates, and in brain by up to 39% at the 10mg/kg dose, compared with untreated alpha-mannosidosis controls. Reductions of up to 60% of urinary mannose containing oligosaccharides were also observed. No histological improvements were seen in the brain at either dose, however marked decreases in lysosomal vacuolation in liver, kidney, spleen and endocrine pancreas, as well as a significant reduction in trigeminal ganglion neurons were observed. Multiple injections of 1mg/kg recombinant enzyme in alpha-mannosidosis guinea-pigs induced a very rapid humoral immune response precluding long-term intravenous treatment.
Keywords: lysosomal storage disease
alpha-mannosidase
animal model
guinea-pig
therapy
enzyme replacement
genetic disease
Description: Copyright © 2006 Elsevier Inc. All rights reserved.
DOI: 10.1016/j.ymgme.2006.05.005
Description (link): http://www.elsevier.com/wps/find/journaldescription.cws_home/622920/description#description
Appears in Collections:Aurora harvest 2
Paediatrics publications

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