Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/23834
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Type: Journal article
Title: Cellulose synthase-like CslF genes mediate the synthesis of cell wall (1,3;1,4)-beta-D-glucans
Author: Burton, R.
Wilson, S.
Hrmova, M.
Harvey, A.
Shirley, N.
Medhurst, A.
Stone, B.
Newbigin, E.
Bacic, A.
Fincher, G.
Citation: Science, 2006; 311(5769):1940-1942
Publisher: Amer Assoc Advancement Science
Issue Date: 2006
ISSN: 0036-8075
1095-9203
Statement of
Responsibility: 
Rachel A. Burton, Sarah M. Wilson, Maria Hrmova, Andrew J. Harvey, Neil J. Shirley, Anne Medhurst, Bruce A. Stone, Edward J. Newbigin, Antony Bacic, Geoffrey B. Fincher
Abstract: A characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-ß-D-glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-ß-D-glucan content in barley grain to a cluster of cellulose synthase–like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis, we detected (1,3;1,4)-ß-D-glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-ß-D-glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-ß-D-glucan biosynthesis.
Keywords: Cell Wall; Plants, Genetically Modified; Arabidopsis; Hordeum; Oryza sativa; Plant Leaves; Glucosyltransferases; beta-Glucans; Antibodies, Monoclonal; Genomics; Transformation, Genetic; Synteny; Genes, Plant; Genome, Plant; Quantitative Trait Loci
RMID: 0020060056
DOI: 10.1126/science.1122975
Appears in Collections:Agriculture, Food and Wine publications

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