Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/23850
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Type: Journal article
Title: Multiple effects of a naturally occurring proline to threonine substitution within acetolactate synthase in two herbicide-resistant populations of Lactuca serriola
Author: Preston, C.
Stone, L.
Rieger, M.
Baker, J.
Citation: Pesticide Biochemistry and Physiology, 2006; 84(3):227-235
Publisher: Academic Press Inc Elsevier Science
Issue Date: 2006
ISSN: 0048-3575
Statement of
Responsibility: 
Christopher Preston, Lynley M. Stone, Mary A. Rieger and Jeanine Baker
Abstract: Two populations of Lactuca serriola L. with resistance to acetolactate synthase (ALS)-inhibiting herbicides were discovered in wheat fields at two locations more than 25 km apart in South Australia. Both resistant populations carried a single base change within a highly conserved coding region of the ALS gene that coded for a single amino acid modification within ALS. The modification of proline 197 to threonine resulted in an enzyme that was highly resistant (>200-fold) to inhibition by sulfonylurea herbicides and moderately resistant to triazolopyrimidine and imidazolinone herbicides. The herbicide-resistant ALS was also less sensitive to inhibition by the branched-chain amino acids valine and leucine. In addition, the resistant enzyme had a lower K{m} for pyruvate. However, extractable ALS activity was similar between resistant and susceptible plants. The substitution of threonine for proline 197 within ALS has multiple impacts on ALS enzyme activity in L. serriola that may influence the frequency of this resistant allele in the environment.
Keywords: Acetohydroxyacid synthase; prickly lettuce; chlorsulfuron; valine; leucine
Rights: Copyright © 2005 Elsevier Inc. All rights reserved.
RMID: 0020060205
DOI: 10.1016/j.pestbp.2005.07.007
Appears in Collections:Agriculture, Food and Wine publications

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