Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/23860
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Type: Journal article
Title: Structure of a flavonoid glucosyltransferase reveals the basis for plant natural product modification
Author: Offen, W.
Martinez-Fleites, C.
Yang, M.
Kiat-Lim, E.
Davis, B.
Tarling, C.
Ford, C.
Bowles, D.
Davies, G.
Citation: EMBO Journal, 2006; 25(6):1396-1405
Publisher: Nature Publishing Group
Issue Date: 2006
ISSN: 0261-4189
1460-2075
Statement of
Responsibility: 
Wendy Offen, Carlos Martinez-Fleites, Min Yang, Eng Kiat-Lim, Benjamin G Davis, Chris A Tarling, Christopher M Ford, Dianna J Bowles, Gideon J Davies
Abstract: Glycosylation is a key mechanism for orchestrating the bioactivity, metabolism and location of small molecules in living cells. In plants, a large multigene family of glycosyltransferases is involved in these processes, conjugating hormones, secondary metabolites, biotic and abiotic environmental toxins, to impact directly on cellular homeostasis. The red grape enzyme UDP-glucose:flavonoid 3-O-glycosyltransferase (VvGT1) is responsible for the formation of anthocyanins, the health-promoting compounds which, in planta, function as colourants determining flower and fruit colour and are precursors for the formation of pigmented polymers in red wine. We show that VvGT1 is active, in vitro, on a range of flavonoids. VvGT1 is somewhat promiscuous with respect to donor sugar specificity as dissected through full kinetics on a panel of nine sugar donors. The three-dimensional structure of VvGT1 has also been determined, both in its 'Michaelis' complex with a UDP-glucose-derived donor and the acceptor kaempferol and in complex with UDP and quercetin. These structures, in tandem with kinetic dissection of activity, provide the foundation for understanding the mechanism of these enzymes in small molecule homeostasis.
Keywords: Catalysis; flavonoid; glycosylation; structure; wine
RMID: 0020060303
DOI: 10.1038/sj.emboj.7600970
Appears in Collections:Agriculture, Food and Wine publications

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