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Type: Journal article
Title: Use of optical density as a measure of Claviceps africana conidial suspension concentration
Author: Herde, D. J.
Ryley, M. J.
Foster, Scott David
Galea, V. J.
Henzell, Robert Philip
Jordan, D. R.
Citation: Australasian Plant Pathology, 2006; 35(1):77-80
Publisher: CSIRO Publishing
Issue Date: 2006
ISSN: 0815-3191
School/Discipline: School of Agriculture, Food and Wine
Statement of
D. J. Herde, M. J. Ryley, S. D. Foster, V. J. Galea, R. G. Henzell and D. R. Jordan
Abstract: Sorghum ergot, caused by Claviceps africana, has remained a major disease problem in Australia since it was first recorded in 1996, and is the focus of a range of biological and integrated management research. Artificial inoculation using conidial suspensions is an important tool in this research. Ergot infection is greatly influenced by environmental factors, so it is important to reduce controllable sources of variation such as inoculum concentration. The use of optical density was tested as a method of quantifying conidial suspensions of C. africana, as an alternative to haemocytometer counts. This method was found to be accurate and time efficient, with possible applications in other disease systems.
Keywords: colorimeter; inoculum concentration; light transmittance; Sorghum bicolor
DOI: 10.1071/AP05093
Appears in Collections:Agriculture, Food and Wine publications

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