Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/23931
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Type: Journal article
Title: Discovery of cyclotide-like protein sequences in graminaceous crop plants: Ancestral precursors of circular proteins?
Author: Mulvenna, J.
Mylne, J.
Bharathi, R.
Burton, R.
Shirley, N.
Fincher, G.
Anderson, M.
Craik, D.
Citation: Plant Cell, 2006; 18(9):2134-2144
Publisher: Amer Soc Plant Physiologists
Issue Date: 2006
ISSN: 1040-4651
1532-298X
Statement of
Responsibility: 
Jason P. Mulvenna, Joshua S. Mylne, Rekha Bharathi, Rachel A. Burton, Neil J. Shirley, Geoffrey B. Fincher, Marilyn A. Anderson and David J. Craik
Abstract: Cyclotides are peptides from plants of the Rubiaceae and Violaceae families that have the unusual characteristic of a macrocylic backbone. They are further characterized by their incorporation of a cystine knot in which two disulfides, along with the intervening backbone residues, form a ring through which a third disulfide is threaded. The cyclotides have been found in every Violaceae species screened to date but are apparently present in only a few Rubiaceae species. The selective distribution reported so far raises questions about the evolution of the cyclotides within the plant kingdom. In this study, we use a combined bioinformatics and expression analysis approach to elucidate the evolution and distribution of the cyclotides in the plant kingdom and report the discovery of related sequences widespread in the Poaceae family, including crop plants such as rice (Oryza sativa), maize (Zea mays), and wheat (Triticum aestivum), which carry considerable economic and social importance. The presence of cyclotide-like sequences within these plants suggests that the cyclotides may be derived from an ancestral gene of great antiquity. Quantitative RT-PCR was used to show that two of the discovered cyclotide-like genes from rice and barley (Hordeum vulgare) have tissue-specific expression patterns.
Keywords: Poaceae; Hordeum; Oryza sativa; Triticum; Zea mays; Crops, Agricultural; Cyclotides; Plant Proteins; Chromosome Mapping; Sequence Alignment; Sequence Analysis, Protein; Computational Biology; Evolution, Molecular; Amino Acid Sequence; Molecular Conformation; Models, Molecular; Molecular Sequence Data
RMID: 0020061276
DOI: 10.1105/tpc.106.042812
Published version: http://www.plantcell.org/cgi/content/abstract/18/9/2134
Appears in Collections:Agriculture, Food and Wine publications

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