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Type: Journal article
Title: Modulation of cholesterol metabolism by the green tea polyphenol (-)-epigallocatechin gallate in cultured human liver (HepG2) cells
Author: Bursill, Christina Anne
Roach, Paul D.
Citation: Journal of Agricultural and Food Chemistry. 54(5):1621-1626
Issue Date: 2006
ISSN: 0021-8561
School/Discipline: School of Molecular and Biomedical Science
Abstract: Epidemiological and animal studies have found that green tea is associated with lower plasma cholesterol. This study aimed to further elucidate how green tea modulates cholesterol metabolism. When HepG2 cells were incubated with the main green tea constituents, the catechins, epigallocatechin gallate (EGCG) was the only catechin to increase LDL receptor binding activity (3-fold) and protein (2.5-fold) above controls. EGCG increased the conversion of sterol regulatory element binding protein-1 (SREBP-1) to its active form (+56%) and lowered the cellular cholesterol concentration (-28%). At 50 M, EGCG significantly lowered cellular cholesterol synthesis, explaining the reduction in cellular cholesterol. At 200 M EGCG, cholesterol synthesis was significantly increased even though cellular cholesterol was lower, but there was a significant increase seen in medium cholesterol. This indicates that, at 200 M, EGCG increases cellular cholesterol efflux. This study provides mechanisms by which green tea modulates cholesterol metabolism and indicates that EGCG might be its active constituent
Keywords: catechins; cholesterol; cholesterol synthesis; lathosterol; SREBP-1
Appears in Collections:Molecular and Biomedical Science publications

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