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https://hdl.handle.net/2440/24067
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dc.contributor.author | Sankovic, N. | - |
dc.contributor.author | Delbridge, M. | - |
dc.contributor.author | Grutzner, F. | - |
dc.contributor.author | Ferguson-Smith, M. | - |
dc.contributor.author | O'Brien, P. | - |
dc.contributor.author | Graves, J. | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | Chromosome Research: the international journal for all aspects of chromosome and nuclear biology, 2006; 14(6):657-664 | - |
dc.identifier.issn | 0967-3849 | - |
dc.identifier.issn | 1573-6849 | - |
dc.identifier.uri | http://hdl.handle.net/2440/24067 | - |
dc.description | © Springer 2006 | - |
dc.description.abstract | The Y chromosome is perhaps the most interesting element of the mammalian genome but comparative analysis of the Y chromosome has been impeded by the difficulty of assembling a shotgun sequence of the Y. BAC-based sequencing has been successful for the human and chimpanzee Y but is difficult to do efficiently for an atypical mammalian model species (Skaletsky et al. 2003, Kuroki et al. 2006). We show how Y-specific sub-libraries can be efficiently constructed using DNA amplified from microdissected or flow-sorted Y chromosomes. A Bacterial Artificial Chromosome (BAC) library was constructed from the model marsupial, the tammar wallaby (Macropus eugenii). We screened this library for Y chromosome-derived BAC clones using DNA from both a microdissected Y chromosome and a flow-sorted Y chromosome in order to create a Y chromosome-specific sub-library. We expected that the tammar wallaby Y chromosome should detect approximately 100 clones from the 2.2 times redundant library. The microdissected Y DNA detected 85 clones, 82% of which mapped to the Y chromosome and the flow-sorted Y DNA detected 71 clones, 48% of which mapped to the Y chromosome. Overall, this represented a approximately 330-fold enrichment for Y chromosome clones. This presents an ideal method for the creation of highly enriched chromosome-specific sub-libraries suitable for BAC-based sequencing of the Y chromosome of any mammalian species. | - |
dc.description.statementofresponsibility | N. Sankovic, M. L. Delbridge, F. Grützner, M. A. Ferguson-Smith, P. C. M. O’Brien and J. A. Marshall Graves | - |
dc.language.iso | en | - |
dc.publisher | Kluwer Academic Publ | - |
dc.source.uri | http://dx.doi.org/10.1007/s10577-006-1076-z | - |
dc.subject | BAC library | - |
dc.subject | Macropus eugenii | - |
dc.subject | tammar wallaby | - |
dc.subject | Y chromosome | - |
dc.title | Construction of a highly enriched marsupial Y chromosome-specific BAC sub-library using isolated Y chromosomes | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1007/s10577-006-1076-z | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Grutzner, F. [0000-0002-3088-7314] | - |
Appears in Collections: | Aurora harvest 2 Environment Institute Leaders publications Molecular and Biomedical Science publications |
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