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|Title:||Microtubules of the mycorrhizal fungus Glomus intraradices in symbiosis with tomato roots|
|Citation:||Botany, 2001; 79(3):307-313|
|Publisher:||Natl Research Council Canada|
|Abstract:||In this study the presence and orientation of fungal microtubules were recorded in arbuscular mycorrhizal symbiosis for the first time. Visualization of the fungal microtubules was achieved by using a protocol specifically labelling only fungal tubulins. Microtubules of external mycelium, intraradical hyphae, arbuscules, and vesicles of the arbuscular mycorrhizal fungus Glomus intraradices Schenck & Smith were examined when in symbiosis with tomato (Lycopersicon esculentum Mill.). Microtubules were organized as bundles in both external and intraradical hyphae. The bundles of microtubules extended directly from intraradical hyphae into the arbuscules, where the microtubules remained as bundles in the larger hyphae. In the fine fungal branches of the arbuscules, microtubules were seen as thinner filaments. Fungal microtubules were seen to connect the intraradical hyphae and arbuscules. In addition, microtubules of adjacent arbuscules could continue directly from one arbuscule to another. Microtubules reached to the basal cone of each vesicle, but the live vesicles, containing many nuclei, seemed devoid of any microtubular labelling.|
|Appears in Collections:||Aurora harvest 2|
Soil and Land Systems publications
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