Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/27489
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Type: Journal article
Title: Modulation of gastro-oesophageal vagal afferents by galanin in mouse and ferret
Author: Page, A.
Slattery, J.
O'Donnell, T.
Isaacs, N.
Young, R.
Blackshaw, L.
Citation: Journal of Physiology-London, 2005; 563(3):809-819
Publisher: Blackwell Publishing Ltd
Issue Date: 2005
ISSN: 0022-3751
1469-7793
Statement of
Responsibility: 
Amanda J. Page, James A. Slattery, Tracey A. O'Donnell, Nicole J. Cooper, Richard L. Young and L. Ashley Blackshaw
Abstract: The neuropeptide galanin is found in the central and peripheral nervous systems. It may have excitatory or inhibitory actions via three subtypes of G-protein-coupled receptor, and it modulates the mechanosensitivity of somatic sensory fibres. We aimed to determine if galanin also modulates vagal afferent mechanosensitivity, and to localize endogenous sources. The responses of ferret and mouse gastro-oesophageal vagal afferents to graded mechanical stimuli were investigated in vitro. The effects of galanin and/or the galanin receptor antagonist galantide on these responses were quantified. Immunohistochemistry for galanin was performed in ferret and mouse proximal stomach and nodose ganglion. In ferrets, retrograde labelling of gastric afferents to the nodose ganglion was combined with immunohistochemistry. When exposed to galanin (1-10 nM), 18/31 ferret and 12/15 mouse gastro-oesophageal afferents (tension, mucosal and tension/mucosal receptors) showed inhibition of mechanosensitivity. Four of 31 ferret afferents showed potentiation of mechanosensitivity, and 9/31 were unaffected (2/15 and 1/15 in mouse, respectively). Galanin effects were reversed after washout or by galantide (10-30 nM). Galantide given alone increased mechanosensitivity. Galanin immunoreactivity was found in nodose neurones, including those innervating the stomach in ferret. Enteric neurones were also galanin immunoreactive, as were endings associated with myenteric ganglia and smooth muscle. We conclude that galanin potently modulates mechanosensitivity of gastro-oesophageal vagal afferents with either facilitatory or inhibitory actions on individual afferent fibres. Both intrinsic and extrinsic (vagal) neurones contain galanin and are therefore potential sources of endogenous galanin.
Keywords: Esophagogastric Junction; Afferent Pathways; Vagus Nerve; Mechanoreceptors; Animals; Ferrets; Mice; Galanin; Mechanotransduction, Cellular; Species Specificity; Tissue Distribution; Dose-Response Relationship, Drug; Female; In Vitro Techniques
Description: Published in Journal of Physiology-London, 2005; 563 (3):809-819 at www.interscience.wiley.com
RMID: 0020050245
DOI: 10.1113/jphysiol.2004.075291
Published version: http://www3.interscience.wiley.com/cgi-bin/fulltext/118735853/PDFSTART
Appears in Collections:Molecular and Biomedical Science publications

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