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Type: Journal article
Title: The two-component signal transduction system RR06/HK06 regulates expression of cbpA in Streptococcus pneumonia
Author: Standish, A.
Stroeher, U.
Paton, J.
Citation: Proceedings of the National Academy of Sciences of USA, 2005; 102(21):7701-7706
Publisher: Natl Acad Sciences
Issue Date: 2005
ISSN: 0027-8424
Statement of
Alistair J. Standish, Uwe H. Stroeher, and James C. Paton
Abstract: Streptococcus pneumoniae encounters a number of environmental niches in the body, including the nasopharynx, lungs, blood, middle ear, and brain. Recent studies have identified 13 putative two-component signal-transduction systems in S. pneumoniae, which are likely to be important for gene regulation in response to external stimuli. Here, we present conclusive evidence for the regulation of choline binding protein A (CbpA), a major pneumococcal virulence factor and protective antigen, by one of these two-component signal-transduction systems. We have demonstrated divergent expression of cbpA in unmarked hk06 and rr06 deletion mutants relative to wild-type S. pneumoniae D39 by using Western immunoblotting and real-time RT-PCR. Electrophoretic mobility-shift and solid-phase binding assays have demonstrated the binding of RR06 to the promoter region of cbpA, suggesting that RR06/HK06 directly regulates cbpA transcription. We have also shown that this system is important for the ability of the pneumococcus to adhere to epithelial cells in vitro and to survive and proliferate in an in vivo mouse model. Thus, the RR06/HK06 system has a significant role in pathogenesis, both in colonization and invasive disease.
Keywords: Epithelial Cells
Streptococcus pneumoniae
Bacterial Proteins
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Electrophoretic Mobility Shift Assay
Reverse Transcriptase Polymerase Chain Reaction
Cell Adhesion
Signal Transduction
Gene Expression Regulation, Bacterial
Promoter Regions, Genetic
Description: © 2005 by The National Academy of Sciences of the USA
DOI: 10.1073/pnas.0409377102
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Appears in Collections:Aurora harvest 2
Molecular and Biomedical Science publications

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