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|Title:||MHC II+ CD45+ cells from synovium-rich tissues of normal rats: phenotype, comparison with macrophage and dendritic cell lineages and differentiation into mature dendritic cells in vitro|
|Citation:||International Immunology, 2005; 17(8):1103-1115|
|Publisher:||Oxford Univ Press|
|Mahin Moghaddami, Leslie G. Cleland and Graham Mayrhofer|
|Abstract:||Synovial tissues are frequent sites of inflammatory disorders in which dendritic cells (DCs) may play an important role. This study examines potential antigen-presenting cells obtained from synovium-rich tissues (SRTs) by vascular perfusion of rat hind limbs with collagenase and further enzymatic digestion of the disarticulated hind paws in vitro. The three sub-populations of interest were: CD45+MHC IIhi, mainly CD11c+ and CD163¯; CD45+MHC II¹°, mainly CD11c¯ and CD163+ and CD45+MHC II¯, mainly CD11c¯ and CD163+. Expression of CD11c and CD163 correlated with ruffled cell-surface (CD11c+CD163¯) and highly vacuolated cytoplasm (CD11c¯CD163+), respectively. Culture of the CD45+CD163¯ sub-population in granulocyte macrophage colony-stimulating factor (GM-CSF) yielded CD45+MHC IIhi CD11c+CD163¯ cells with veiled morphology, while the large vacuolated cells that expressed CD163 resembled type A synoviocytes in both surface antigen phenotype and morphology. These results demonstrate that SRTs contain indeterminate cells that can differentiate into mature DCs in vitro in response to GM-CSF, plus mature synovial lining macrophages.|
|Keywords:||animal model; antigen-presenting cells; cytokines; polyarthritis; synovial cells|
|Description:||Copyright © 2005 Japanese Society for Immunology|
|Appears in Collections:||Molecular and Biomedical Science publications|
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