Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/27576
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Type: Journal article
Title: Essential roles of sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells
Author: Pebay, A.
Wong, R.
Pitson, S.
Wolvetang, E.
Peh, G.
Filipczyk, A.
Koh, K.
Tellis, I.
Nguyen, L.
Pera, M.
Citation: Stem Cells, 2005; 23(10):1541-1548
Publisher: Alphamed Press
Issue Date: 2005
ISSN: 1066-5099
1549-4918
Abstract: Human embryonic stem cells (hESCs) have great potential for use in research and regenerative medicine, but very little is known about the factors that maintain these cells in the pluripotent state. We investigated the role of three major mitogenic agents present in serum--sphingosine-1-phosphate (S1P), lysophosphatidic acid (LPA), and platelet-derived growth factor (PDGF)--in maintaining hESCs. We show here that although LPA does not affect hESC growth or differentiation, coincubation of S1P and PDGF in a serum-free culture medium successfully maintains hESCs in an undifferentiated state. Our studies indicate that signaling pathways activated by tyrosine kinase receptors act synergistically with those downstream from lysophospholipid receptors to maintain hESCs in the undifferentiated state. This study is the first demonstration of a role for lysophospholipid receptor signaling in the maintenance of stem cell pluri-potentiality.
Keywords: Cells, Cultured; Stem Cells; Humans; Sphingosine; Receptor, Platelet-Derived Growth Factor beta; Lysophospholipids; Platelet-Derived Growth Factor; Receptors, Lysophosphatidic Acid; Cell Culture Techniques; Embryo Research; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction
RMID: 0020051369
DOI: 10.1634/stemcells.2004-0338
Appears in Collections:Molecular and Biomedical Science publications

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