Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/27609
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dc.contributor.authorPralong, D.en
dc.contributor.authorLim, M.en
dc.contributor.authorVassiliev, I.en
dc.contributor.authorMrozik, K.en
dc.contributor.authorWijesundara, N.en
dc.contributor.authorRathjen, P.en
dc.contributor.authorVerma, P.en
dc.date.issued2005en
dc.identifier.citationCloning and Stem Cells, 2005; 7(4):272-278en
dc.identifier.issn1536-2302en
dc.identifier.issn1557-7457en
dc.identifier.urihttp://hdl.handle.net/2440/27609-
dc.description.abstractThe demonstration that mouse somatic cells can be reprogrammed following fusion with embryonic stem (ES) cells may provide an alternative to somatic cell nuclear transfer (therapeutic cloning) to generate autologous stem cells. In an attempt to produce cells with an increased pool of reprogramming factors, tetraploid ES cells were produced by polyethylene glycol mediated fusion of two ES cell lines transfected with plasmids carrying puromycin or neomycin resistance cassettes, respectively, followed by double antibiotic selection. Tetraploid ES cells retain properties characteristic of diploid ES cells, including the expression of pluripotent gene markers Oct4 and Rex1. On injection into the testis capsule of severe combined immunodeficient (SCID) mice, tetraploid ES cells are able to form teratomas containing cells representative of all three germ layers. Further, these cells demonstrated the ability to integrate into the inner cell mass of blastocysts. This study indicates that tetraploid ES cells are promising candidates as cytoplasm donors for reprogramming studies.en
dc.description.statementofresponsibilityDaniele Pralong, Mei Ling Lim, Ivan Vassiliev, Krzysztof Mrozik, Nishanthi Wijesundara, Peter Rathjen, Paul J. Vermaen
dc.description.urihttp://www.ncbi.nlm.nih.gov/pubmed/16390263en
dc.language.isoenen
dc.publisherMary Ann Liebert, Inc. Publishersen
dc.subjectTestis; Cells, Cultured; Stem Cells; Blastocyst; Germ Layers; Animals; Mice; Mice, SCID; Teratoma; Stem Cell Transplantation; Polyploidy; Male; Octamer Transcription Factor-3en
dc.titleTetraploid embryonic stem cells contribute to the inner cell mass of mouse blastocystsen
dc.typeJournal articleen
dc.identifier.rmid0020052734en
dc.contributor.organisationCentre for the Molecular Genetics of Developmenten
dc.identifier.doi10.1089/clo.2005.7.272en
dc.identifier.pubid53430-
pubs.library.collectionMolecular and Biomedical Science publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidMrozik, K. [0000-0002-4890-8208]en
Appears in Collections:Molecular and Biomedical Science publications
Centre for the Molecular Genetics of Development publications

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