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|Web of Science®
|Rhizobium meliloti lacking mosA synthesizes the rhizopine scyllo-inosamine in place of 3-O-methyl-scyllo-inosamine
|Rao, J. Papa
Murphy, Peter J.
|Microbiology, 1995; 141(7):1683-1690
|Society for General Microbiology
|School of Agriculture, Food and Wine : Plant and Food Science
School of Agriculture, Food and Wine
|J. Papa Rao, Wojciech Grzemski and Peter J. Murphy
|Summary: The Rhizobium meliloti Rm220-3 mos locus producing the rhizopine scyllo-inosamine (SI) in nodules is shown to consist of three ORFs (ORF1, mosB and mosC) arranged in an operon structure. This differs from the R. meliloti L5-30 mos locus, which produces 3-O-methyl-scyllo-inosamine (3-O-MSI), by the complete absence of mosA. The deletion covers a region of 1235 nt and includes the entire mosA gene as well as the sequence both upstream and downstream. As a result, Rm220-3 mos ORF1 shares a 5′ region common with L5-30 ORF1 but includes an additional 10 bp insertion and a section in the L5-30 mosA and mosB intercistronic region. Antibodies against L5-30 Mos proteins detected MosB and MosC proteins in Rm220-3-induced nodules but no translation product for either ORF1 or mosA was detected. A construct prepared from the L5-30 mos locus which has a truncated mosA gene produces SI rather than 3-O-MSI, confirming this gene is involved in a methylation step in the production of 3-O-MSI. Further, changes made to this ORF result in reduced levels of the rhizopine.
|rhizopine synthesis genes; Rhizobium meliloti; symbiosis
|© 1995 Society for General Microbiology
|Appears in Collections:
|Agriculture, Food and Wine publications
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