Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/27915
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Type: Journal article
Title: DNA fingerprinting probe specific to isolates of Rhizoctonia solani AG-3
Author: Balali, G.
Whisson, D.
Scott, E.
Neate, S.
Citation: Mycological Research, 1996; 100(4):467-470
Publisher: CAMBRIDGE UNIV PRESS
Issue Date: 1996
ISSN: 0953-7562
Abstract: Anastomosis group 3 (AG-3) isolates of Rhizoctonia solani that cause black scurf were recovered from diseased potatoes in Virginia and Lenswood in South Australia. A highly repeated and specific DNA fingerprinting probe, pR3718, prepared from AG-3 isolate R37, hybridized to Southem blots of all AG-3 isolates tested, but had no or very weak reactions with isolates from other anastomosis groups. Genetic variation among the isolates was observed using the fingerprinting probe. Sac I or Hind III restriction endonuclease digests of DNA prepared from 136 AG-3 isolates revealed 38 fingerprint patterns, each containing 12-19 Sac I or Hind III fragments. Because the pR3718 clone recognizes a DNA sequence with a high number of copies per genome and appears to be specific for AG-3 isolates of Rhizoctonia, it has the potential to be used as a diagnostic tool to study the epidemiology, genetic diversity and population dynamics of this important plant pathogen.
RMID: 0030002560
DOI: 10.1016/S0953-7562(96)80145-6
Appears in Collections:Agriculture, Food and Wine publications

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